Skip to main content

Mchaourab Lab Publications

2016

Zhu S , Stein RA , Yoshioka C , Lee CH , Goehring A , Mchaourab HS , Gouaux E , Mechanism of NMDA Receptor Inhibition and Activation. Cell. 2016 Apr 6;
Arrigoni C , Rohaim A , Shaya D , Findeisen F , Stein RA , Nurva SR , Mishra S , Mchaourab HS , Minor DL , Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation. Cell. 2016 Feb 25; 164 (5). 922-36
Khantwal CM , Abraham SJ , Han W , Jiang T , Chavan TS , Cheng RC , Elvington SM , Liu CW , Mathews II , Stein RA , Mchaourab HS , Tajkhorshid E , Maduke M , Revealing an outward-facing open conformational state in a CLC Cl(-)/H(+) exchange transporter. eLife. 2016 ; 5
Dastvan R , Fischer AW , Mishra S , Meiler J , Mchaourab HS , Protonation-dependent conformational dynamics of the multidrug transporter EmrE. Proceedings of the National Academy of Sciences of the United States of America. 2016 Feb 2; 113 (5). 1220-5
Krug U , Alexander NS , Stein RA , Keim A , Mchaourab HS , Sträter N , Meiler J , Characterization of the Domain Orientations of E. coli 5′-Nucleotidase by Fitting an Ensemble of Co… Structure (London, England : 1993). 2016 Jan 5; 24 (1). 43-56

2015

Koteiche HA , Claxton DP , Mishra S , Stein RA , McDonald ET , Mchaourab HS , Species-Specific Structural and Functional Divergence of α-Crystallins: Zebrafish αBa- and Rodent … Biochemistry. 2015 Sep 29; 54 (38). 5949-58
Zou P , Wu SY , Koteiche HA , Mishra S , Levic DS , Knapik E , Chen W , Mchaourab HS , A conserved role of αA-crystallin in the development of the zebrafish embryonic lens. Experimental eye research. 2015 Sep ; 138 104-13
Claxton DP , Kazmier K , Mishra S , Mchaourab HS , Navigating Membrane Protein Structure, Dynamics, and Energy Landscapes Using Spin Labeling and EPR S… Methods in enzymology. 2015 ; 564 349-87
Mori T , Mchaourab H , Johnson CH , Circadian Clocks: Unexpected Biochemical Cogs. Current biology : CB. 2015 Oct 5; 25 (19). R842-4
Schmandt N , Velisetty P , Chalamalasetti SV , Stein RA , Bonner R , Talley L , Parker MD , Mchaourab HS , Yee VC , Lodowski DT , Chakrapani S , A chimeric prokaryotic pentameric ligand-gated channel reveals distinct pathways of activation. The Journal of general physiology. 2015 Oct ; 146 (4). 323-40
Kaur H , Lakatos A , Spadaccini R , Vogel R , Hoffmann C , Becker-Baldus J , Ouari O , Tordo P , Mchaourab H , Glaubitz C , The ABC exporter MsbA probed by solid state NMR – challenges and opportunities. Biological chemistry. 2015 Sep ; 396 (9-10). 1135-49
Meyer V , Swanson MA , Clouston LJ , Boratyński PJ , Stein RA , Mchaourab HS , Rajca A , Eaton SS , Eaton GR , Room-temperature distance measurements of immobilized spin-labeled protein by DEER/PELDOR. Biophysical journal. 2015 Mar 10; 108 (5). 1213-9
Anderson DM , Floyd KA , Barnes S , Clark JM , Clark JI , Mchaourab H , Schey KL , A method to prevent protein delocalization in imaging mass spectrometry of non-adherent tissues: app… Analytical and bioanalytical chemistry. 2015 Mar ; 407 (8). 2311-20

2014

Kazmier K , Sharma S , Islam SM , Roux B , Mchaourab HS , Conformational cycle and ion-coupling mechanism of the Na+/hydantoin transporter Mhp1. Proceedings of the National Academy of Sciences of the United States of America. 2014 Oct 14; 111 (41). 14752-7
Yu Z , Quine RW , Rinard GA , Tseitlin M , Elajaili H , Kathirvelu V , Clouston LJ , Boratyński PJ , Rajca A , Stein R , Mchaourab H , Eaton SS , Eaton GR , Rapid-scan EPR of immobilized nitroxides. Journal of magnetic resonance (San Diego, Calif. : 1997). 2014 Oct ; 247 67-71
Dürr KL , Chen L , Stein RA , De Zorzi R , Folea IM , Walz T , Mchaourab HS , Gouaux E , Structure and dynamics of AMPA receptor GluA2 in resting, pre-open, and desensitized states. Cell. 2014 Aug 14; 158 (4). 778-92
Mishra S , Verhalen B , Stein RA , Wen PC , Tajkhorshid E , Mchaourab HS , Conformational dynamics of the nucleotide binding domains and the power stroke of a heterodimeric AB… eLife. 2014 ; 3 e02740
Kazmier K , Sharma S , Quick M , Islam SM , Roux B , Weinstein H , Javitch JA , McHaourab HS , Conformational dynamics of ligand-dependent alternating access in LeuT. Nature structural & molecular biology. 2014 May ; 21 (5). 472-9
Masureel M , Martens C , Stein RA , Mishra S , Ruysschaert JM , Mchaourab HS , Govaerts C , Protonation drives the conformational switch in the multidrug transporter LmrP. Nature chemical biology. 2014 Feb ; 10 (2). 149-55

2013

Hamilton PJ , Campbell NG , Sharma S , Erreger K , Herborg Hansen F , Saunders C , Belovich AN , , Sahai MA , Cook EH , Gether U , McHaourab HS , Matthies HJ , Sutcliffe JS , Galli A , De novo mutation in the dopamine transporter gene associates dopamine dysfunction with autism spectr… Molecular psychiatry. 2013 Dec ; 18 (12). 1315-23
Steed PR , Stein RA , Mishra S , Goodman MC , McHaourab HS , Na⁺-substrate coupling in the multidrug antiporter norm probed with a spin-labeled substrate. Biochemistry. 2013 Aug 27; 52 (34). 5790-9
Wen PC , Verhalen B , Wilkens S , Mchaourab HS , Tajkhorshid E , On the origin of large flexibility of P-glycoprotein in the inward-facing state. The Journal of biological chemistry. 2013 Jun 28; 288 (26). 19211-20
Islam SM , Stein RA , McHaourab HS , Roux B , Structural refinement from restrained-ensemble simulations based on EPR/DEER data: application to T4… The journal of physical chemistry. B. 2013 May 2; 117 (17). 4740-54
Shi J , Koteiche HA , McDonald ET , Fox TL , Stewart PL , McHaourab HS , Cryoelectron microscopy analysis of small heat shock protein 16.5 (Hsp16.5) complexes with T4 lysozy… The Journal of biological chemistry. 2013 Feb 15; 288 (7). 4819-30
Hamilton PJ , Campbell NG , Sharma S , Erreger K , Hansen FH , Saunders C , Belovich AN , Sahai MA , Cook EH , Gether U , McHaourab HS , Matthies HJ , Sutcliffe JS , Galli A , Drosophila melanogaster: a novel animal model for the behavioral characterization of autism-associat… Molecular psychiatry. 2013 Dec ; 18 (12). 1235
Steed PR , Zou P , Trone KE , Mchaourab HS , Structure and pH-induced structural rearrangements of the putative multidrug efflux pump EmrD in lip… Biochemistry. 2013 Nov 12; 52 (45). 7964-74
Alexander NS , Stein RA , Koteiche HA , Kaufmann KW , McHaourab HS , Meiler J , RosettaEPR: rotamer library for spin label structure and dynamics. PloS one. 2013 ; 8 (9). e72851

2012

Mishra S , Stein RA , McHaourab HS , Cataract-linked γD-crystallin mutants have weak affinity to lens chaperones α-crystallins. FEBS letters. 2012 Feb 17; 586 (4). 330-6
Mishra S , Stein RA , Mchaourab HS , Cataract-linked γD-crystallin mutants have weak affinity to lens chaperones α-crystallins FEBS Letters. 2012 Jan 29; 4 (586). 330-6
Yirdaw RB , McHaourab HS , Direct observation of T4 lysozyme hinge-bending motion by fluorescence correlation spectroscopy. Biophysical journal. 2012 Oct 3; 103 (7). 1525-36
McHaourab HS , Lin YL , Spiller BW , Crystal structure of an activated variant of small heat shock protein Hsp16.5. Biochemistry. 2012 Jun 26; 51 (25). 5105-12
McDonald ET , Bortolus M , Koteiche HA , Mchaourab HS , Sequence, structure, and dynamic determinants of Hsp27 (HspB1) equilibrium dissociation are encoded … Biochemistry. 2012 Feb 14; 51 (6). 1257-68

2011

McHaourab HS , Steed PR , Kazmier K , Toward the fourth dimension of membrane protein structure: insight into dynamics from spin-labeling … Structure (London, England : 1993). 2011 Nov 9; 19 (11). 1549-61
Mushrush DJ , Koteiche HA , Sammons MA , Link AJ , McHaourab HS , Lacy DB , Studies of the mechanistic details of the pH-dependent association of botulinum neurotoxin with memb… The Journal of biological chemistry. 2011 Jul 29; 286 (30). 27011-8
Hoffman L , Stein RA , Colbran RJ , Mchaourab HS , Conformational changes underlying calcium/calmodulin-dependent protein kinase II activation. The EMBO journal. 2011 Apr 6; 30 (7). 1251-62
Kazmier K , Alexander NS , Meiler J , McHaourab HS , Algorithm for selection of optimized EPR distance restraints for de novo protein structure determina… Journal of structural biology. 2011 Mar ; 173 (3). 549-57
Hirst SJ , Alexander N , McHaourab HS , Meiler J , RosettaEPR: an integrated tool for protein structure determination from sparse EPR data. Journal of structural biology. 2011 Mar ; 173 (3). 506-14

2010

Tycko R , Savtchenko R , Ostapchenko VG , Makarava N , Baskakov IV , The α-helical C-terminal domain of full-length recombinant PrP converts to an in-register parallel … Biochemistry. 2010 Nov 9; 49 (44). 9488-97
Qin X , Byrne M , Mori T , Zou P , Williams DR , McHaourab H , Johnson CH , Intermolecular associations determine the dynamics of the circadian KaiABC oscillator. Proceedings of the National Academy of Sciences of the United States of America. 2010 Aug 17; 107 (33). 14805-10
Claxton DP , Quick M , Shi L , de Carvalho FD , Weinstein H , Javitch JA , McHaourab HS , Ion/substrate-dependent conformational dynamics of a bacterial homolog of neurotransmitter:sodium sy… Nature structural & molecular biology. 2010 Jul ; 17 (7). 822-9
Amadi ST , Koteiche HA , Mishra S , McHaourab HS , Structure, dynamics, and substrate-induced conformational changes of the multidrug transporter EmrE … The Journal of biological chemistry. 2010 Aug 20; 285 (34). 26710-8
Gustot A , Smriti , Ruysschaert JM , McHaourab H , Govaerts C , Lipid composition regulates the orientation of transmembrane helices in HorA, an ABC multidrug trans… The Journal of biological chemistry. 2010 May 7; 285 (19). 14144-51

2009

Zou P , Bortolus M , McHaourab HS , Conformational cycle of the ABC transporter MsbA in liposomes: detailed analysis using double electr… Journal of molecular biology. 2009 Oct 30; 393 (3). 586-97
McHaourab HS , Godar JA , Stewart PL , Structure and mechanism of protein stability sensors: chaperone activity of small heat shock protein… Biochemistry. 2009 May 12; 48 (18). 3828-37
Smriti P , Zou HS , McHaourab , Mapping daunorubicin-binding Sites in the ATP-binding cassette transporter MsbA using site-specific … The Journal of biological chemistry. 2009 May 15; 284 (20). 13904-13
Latham JC , Stein RA , Bornhop DJ , Mchaourab HS , Free-solution label-free detection of alpha-crystallin chaperone interactions by back-scattering int… Analytical chemistry. 2009 Mar 1; 81 (5). 1865-71
Kumar MS , Koteiche HA , Claxton DP , Mchaourab HS , Disulfide cross-links in the interaction of a cataract-linked alphaA-crystallin mutant with betaB1-c… FEBS letters. 2009 Jan 5; 583 (1). 175-9
Zou P , McHaourab HS , Alternating access of the putative substrate-binding chamber in the ABC transporter MsbA. Journal of molecular biology. 2009 Oct 30; 393 (3). 574-85

2008

McHaourab HS , Mishra S , Koteiche HA , Amadi SH , Role of sequence bias in the topology of the multidrug transporter EmrE. Biochemistry. 2008 Aug 5; 47 (31). 7980-2
Alexander N , Bortolus M , Al-Mestarihi A , Mchaourab H , Meiler J , De novo high-resolution protein structure determination from sparse spin-labeling EPR data. Structure (London, England : 1993). 2008 Feb ; 16 (2). 181-95
Claxton DP , Zou P , Mchaourab HS , Structure and orientation of T4 lysozyme bound to the small heat shock protein alpha-crystallin. Journal of molecular biology. 2008 Jan 25; 375 (4). 1026-39

2007

Cobb NJ , Sönnichsen FD , McHaourab H , Surewicz WK , Molecular architecture of human prion protein amyloid: a parallel, in-register beta-structure. Proceedings of the National Academy of Sciences of the United States of America. 2007 Nov 27; 104 (48). 18946-51
Borbat PP , Surendhran K , Bortolus M , Zou P , Freed JH , Mchaourab HS , Conformational motion of the ABC transporter MsbA induced by ATP hydrolysis. PLoS biology. 2007 Oct ; 5 (10). e271
McHaourab HS , Kumar MS , Koteiche HA , Specificity of alphaA-crystallin binding to destabilized mutants of betaB1-crystallin. FEBS letters. 2007 May 15; 581 (10). 1939-43
Koteiche HA , Kumar MS , McHaourab HS , Analysis of betaB1-crystallin unfolding equilibrium by spin and fluorescence labeling: evidence of a… FEBS letters. 2007 May 15; 581 (10). 1933-8
Mori T , Williams DR , Byrne MO , Qin X , Egli M , Mchaourab HS , Stewart PL , Johnson CH , Elucidating the ticking of an in vitro circadian clockwork. PLoS biology. 2007 Apr ; 5 (4). e93
Zou P , Surendhran K , Mchaourab HS , Distance measurements by fluorescence energy homotransfer: evaluation in T4 lysozyme and correlation… Biophysical journal. 2007 Feb 15; 92 (4). L27-9

2006

Shi J , Koteiche HA , McHaourab HS , Stewart PL , Cryoelectron microscopy and EPR analysis of engineered symmetric and polydisperse Hsp16.5 assemblies… The Journal of biological chemistry. 2006 Dec 29; 281 (52). 40420-8
Koteiche HA , Mchaourab HS , Mechanism of a hereditary cataract phenotype. Mutations in alphaA-crystallin activate substrate bind… The Journal of biological chemistry. 2006 May 19; 281 (20). 14273-9

2005

Koteiche HA , Chiu S , Majdoch RL , Stewart PL , Mchaourab HS , Atomic models by cryo-EM and site-directed spin labeling: application to the N-terminal region of Hs… Structure (London, England : 1993). 2005 Aug ; 13 (8). 1165-71
Altenbach C , Froncisz W , Hemker R , McHaourab H , Hubbell WL , Accessibility of nitroxide side chains: absolute Heisenberg exchange rates from power saturation EPR… Biophysical journal. 2005 Sep ; 89 (3). 2103-12
Dong J , Yang G , McHaourab HS , Structural basis of energy transduction in the transport cycle of MsbA. Science (New York, N.Y.). 2005 May 13; 308 (5724). 1023-8
Shashidharamurthy R , Koteiche HA , Dong J , McHaourab HS , Mechanism of chaperone function in small heat shock proteins: dissociation of the HSP27 oligomer is … The Journal of biological chemistry. 2005 Feb 18; 280 (7). 5281-9

2004

Sathish HA , Koteiche HA , McHaourab HS , Binding of destabilized betaB2-crystallin mutants to alpha-crystallin: the role of a folding interme… The Journal of biological chemistry. 2004 Apr 16; 279 (16). 16425-32

2003

Sathish HA , Stein RA , Yang G , Mchaourab HS , Mechanism of chaperone function in small heat-shock proteins. Fluorescence studies of the conformati… The Journal of biological chemistry. 2003 Nov 7; 278 (45). 44214-21
Koteiche HA , Reeves MD , McHaourab HS , Structure of the substrate binding pocket of the multidrug transporter EmrE: site-directed spin labe… Biochemistry. 2003 May 27; 42 (20). 6099-105
Koteiche HA , McHaourab HS , Mechanism of chaperone function in small heat-shock proteins. Phosphorylation-induced activation of … The Journal of biological chemistry. 2003 Mar 21; 278 (12). 10361-7

2002

Mchaourab HS , Dodson EK , Koteiche HA , Mechanism of chaperone function in small heat shock proteins. Two-mode binding of the excited states… The Journal of biological chemistry. 2002 Oct 25; 277 (43). 40557-66
Koteiche HA , Mchaourab HS , The determinants of the oligomeric structure in Hsp16.5 are encoded in the alpha-crystallin domain. FEBS letters. 2002 May 22; 519 (1-3). 16-22
Borbat PP , McHaourab HS , Freed JH , Protein structure determination using long-distance constraints from double-quantum coherence ESR: s… Journal of the American Chemical Society. 2002 May 15; 124 (19). 5304-14
Mansoor SE , McHaourab HS , Farrens DL , Mapping proximity within proteins using fluorescence spectroscopy. A study of T4 lysozyme showing th… Biochemistry. 2002 Feb 26; 41 (8). 2475-84

2000

Kaplan RS , Mayor JA , Kotaria R , Walters DE , McHaourab HS , The yeast mitochondrial citrate transport protein: determination of secondary structure and solvent … Biochemistry. 2000 Aug 8; 39 (31). 9157-63
Haley DA , Bova MP , Huang QL , Mchaourab HS , Stewart PL , Small heat-shock protein structures reveal a continuum from symmetric to variable assemblies. Journal of molecular biology. 2000 Apr 28; 298 (2). 261-72
Bova MP , McHaourab HS , Han Y , Fung BK , Subunit exchange of small heat shock proteins. Analysis of oligomer formation of alphaA-crystallin a… The Journal of biological chemistry. 2000 Jan 14; 275 (2). 1035-42

1999

Koteiche HA , Mchaourab HS , Folding pattern of the alpha-crystallin domain in alphaA-crystallin determined by site-directed spin… Journal of molecular biology. 1999 Nov 26; 294 (2). 561-77
Mansoor SE , McHaourab HS , Farrens DL , Determination of protein secondary structure and solvent accessibility using site-directed fluoresce… Biochemistry. 1999 Dec 7; 38 (49). 16383-93
Barnes JP , Liang Z , Mchaourab HS , Freed JH , Hubbell WL , A multifrequency electron spin resonance study of T4 lysozyme dynamics. Biophysical journal. 1999 Jun ; 76 (6). 3298-306
Mchaourab HS , Kálai T , Hideg K , Hubbell WL , Motion of spin-labeled side chains in T4 lysozyme: effect of side chain structure. Biochemistry. 1999 Mar 9; 38 (10). 2947-55
Berengian AR , Parfenova M , Mchaourab HS , Site-directed spin labeling study of subunit interactions in the alpha-crystallin domain of small he… The Journal of biological chemistry. 1999 Mar 5; 274 (10). 6305-14

1998

Koteiche HA , Berengian AR , Mchaourab HS , Identification of protein folding patterns using site-directed spin labeling. Structural characteriz… Biochemistry. 1998 Sep 15; 37 (37). 12681-8

1997

Mchaourab HS , Oh KJ , Fang CJ , Hubbell WL , Conformation of T4 lysozyme in solution. Hinge-bending motion and the substrate-induced conformation… Biochemistry. 1997 Jan 14; 36 (2). 307-16
Mchaourab HS , Berengian AR , Koteiche HA , Site-directed spin-labeling study of the structure and subunit interactions along a conserved sequen… Biochemistry. 1997 Dec 2; 36 (48). 14627-34
Berengian AR , Bova MP , Mchaourab HS , Structure and function of the conserved domain in alphaA-crystallin. Site-directed spin labeling ide… Biochemistry. 1997 Aug 19; 36 (33). 9951-7
Qu K , Vaughn JL , Sienkiewicz A , Scholes CP , Fetrow JS , Kinetics and motional dynamics of spin-labeled yeast iso-1-cytochrome c: 1. Stopped-flow electron pa… Biochemistry. 1997 Mar 11; 36 (10). 2884-97

1996

Hubbell WL , Mchaourab HS , Altenbach C , Lietzow MA , Watching proteins move using site-directed spin labeling. Structure (London, England : 1993). 1996 Jul 15; 4 (7). 779-83
Mchaourab HS , Lietzow MA , Hideg K , Hubbell WL , Motion of spin-labeled side chains in T4 lysozyme. Correlation with protein structure and dynamics. Biochemistry. 1996 Jun 18; 35 (24). 7692-704

1995

Singh RJ , Feix JB , Mchaourab HS , Hogg N , Kalyanaraman B , Spin-labeling study of the oxidative damage to low-density lipoprotein. Archives of biochemistry and biophysics. 1995 Jun 20; 320 (1). 155-61

1994

Singh RJ , Hogg N , Mchaourab HS , Kalyanaraman B , Physical and chemical interactions between nitric oxide and nitroxides. Biochimica et biophysica acta. 1994 Dec 15; 1201 (3). 437-41
Mchaourab HS , Hyde JS , Feix JB , Binding and state of aggregation of spin-labeled cecropin AD in phospholipid bilayers: effects of su… Biochemistry. 1994 May 31; 33 (21). 6691-9

1993

Mchaourab HS , Hyde JS , Feix JB , Aggregation state of spin-labeled cecropin AD in solution. Biochemistry. 1993 Nov 9; 32 (44). 11895-902
Mchaourab HS , Pfenninger S , Antholine WE , Felix CC , Hyde JS , Kroneck PM , Multiquantum EPR of the mixed valence copper site in nitrous oxide reductase. Biophysical journal. 1993 May ; 64 (5). 1576-9