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Gould Lab

Gould Lab Publications

2024

Tavafoghi B , Ren L , Gould KL , Willet AH , Generation and characterization of temperature-sensitive alleles encoding GPI anchored proteins Psu1 and Dfg502 in Schizosaccharomyces pombe microPublication Biology. 2024 Apr 2;
Iglesias-Ronero AB , Soto T , Flor-Parra I , Salas-Pino S , Ruiz-Romero G , Gould KL , Cansado J , Daga RR , MAPK-dependent control of mitotic progression in S. pombe BMC Biology. 2024 Mar 25; 71 (22).
Howard I.V. , Tavafoghi B , Igarashi M.G. , Ren L , Willet A.H. , Gould K.L. , Generation and characterization of temperature-sensitive alleles of the glucanosyltransferase Gas1 in Schizosaccharomyces pombe microPublication Biology. 2024 Mar 5;
Maury LL , Ren L , Hassan S , Bahler J , Gould KL , The Cdc14 phosphatase, Clp1, does not affect genome expression microPublication Biology. 2024 Feb ;

2023

Willet AH , Turner LA , Park JS , Ren L , Snider CE , Gould KL , Characterization of Pik1 function in fission yeast reveals its conserved role in lipid synthesis and not cytokinesis Journal of Cell Science. 2023 Oct 10;
Willet AH , Wos M , Igarashi MG , Ren L , Turner LA , Gould KL , Elevated levels of sphingolipid MIPC in the plasma membrane disrupt the coordination of cell growth with cell wall formation in fission yeast PLoS Genetics. 2023 Oct 4;
Lera-Ramirez M , Bahler J , Mata J , Rutherford K , Hoffman C , Lambert S , Oliferenko S , Martin SG , Gould KL , Du L , Sabatinos S , Forsburg SL , Nielsen O , Nurse P , Wood V , Revised fission yeast gene and allele nomenclature guidelines for machine readability Genetics. 2023 Sep 27;
Igrashi MG , Bhattacharjee R , Willet AH , Gould KL , Polarity kinases that phosphorylate F-BAR protein Cdc15 have unique localization patterns during cytokinesis and contributions to preventing tip septation in Schizosaccharomyces pombe microPublication Biology. 2023 Sep ;
Willet AH , Ren L , Turner LA , Gould KL , Isolation of mutant alleles of the U6 snRNA m6A methyltransferase Mtl16 and characterization of their genetic interactions with splicing mutants in Schizosaccharomyces pombe microPublication Biology. 2023 Aug 11;
Willet AH , Chen JS , Ren L , Gould KL , Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins Molecular Biology of the Cell. 2023 Aug 2;
Rossi AR , Bohnert KA , Gould KL , The fission yeast cytokinetic ring component Fic1 promotes septum formation Biology Open. 2023 May ; 5 (12).
Bhattacharjee R , Hall A , Mangione M , Igarashi M , Roberts-Galbraith R , Chen JS , Vavylonis D , Gould KL , Multiple polarity kinases inhibit phase separation of F-BAR protein Cdc15 and antagonize cytokinetic ring assembly in fission yeast eLife. 2023 Feb 7;

2022

Cullati SN , Chaikuad A , Chen JS , Grebel J , Tesmer L , Zhubi R , Navarrete-perea J , Guillen RX , Gygi SP , Hummer G , Dotsch V , Knapp S , Gould KL , Kinase domain autophosphorylation rewires the activity and substrate specificity of CK1 enzymes Molecular Cell. 2022 Mar 29;
Snider CE* , Bhattacharjee R* , Igarashi MG , Gould KL , Fission yeast paxillin contains two Cdc15 binding motifs for robust recruitment to the cytokinetic ring Molecular Biology of the Cell. 2022 Feb 2; 33 (4).

2021

Chen JS , Jones CM , Igarashi MG , Ren L , Johnson AE , Gould KL , Localization of the ubiquitin ligase Dma1 to the fission yeast contractile ring is modulated by phosphorylation FEBS Letters. 2021 Nov 2; (595). 2781–92
Willet AH , Igarashi MG , Chen JS , Bhattacharjee R , Ren L , Cullati SN , Elmore ZC , Roberts-Galbraith RH , Johnson AE , Beckley JR , Gould KL , Phosphorylation in the intrinsically disordered region of F-BAR protein Imp2 regulates its contractile ring recruitment Journal of Cell Science. 2021 Aug 17; 134 (16).
Mangione MC , Chen JS , Gould KL , Cdk1 phosphorylation of fission yeast paxillin inhibits its cytokinetic ring localization Molecular Biology of the Cell. 2021 Jun 16; 32 (17). 1534-44
Snider CE , Wan Mohamad Noor WNI , Nguyen NTH , Gould KL , Suetsugu S , The state of F-BAR domains as membrane-bound oligomeric platforms Trends in Cell Biology. 2021 Aug 20; 31 (8). 644-55

2020

Snider CE , Chandra M , McDonald N , Willet AH , Collier S , Ohi MD , Jackson LP , Gould KL , Opposite Surfaces of the Cdc15 F-BAR Domain Create a Membrane Platform That Coordinates Cytoskeletal and Signaling Components for Cytokinesis Cell Reports. 2020 Dec 22; 33 (12).
Snider CE , Willet AH , Brown HT , Chen JS , Evers JM , Gould KL , Fission yeast Opy1 is an endogenous PI(4,5)P sensor that binds the PI5-kinase Its3 Journal of cell science. 2020 Dec 3; 133 (23).
Bohnert KA , Rossi AM , Jin QW , Chen JS , Gould KL , Phosphoregulation of the Cytokinetic Protein Fic1 Contributes to Fission Yeast Growth Polarity Establishment Journal of cell science. 2020 Sep 2; 133 (18).
Guillen RX , Beckley JR , Chen JS , Gould KL , CRISPR-mediated gene targeting of CK1δ/ε leads to enhanced understanding of their role in endocytosis via phosphoregulation of GAPVD1 Scientific reports. 2020 Apr 22; 10 (1). 6797
Bhattacharjee R , Mangione MC , Wos M , Chen JS , Snider CE , Roberts-Galbraith RH , McDonald NA , Presti LL , Martin SG , Gould KL , DYRK kinase Pom1 drives F-BAR protein Cdc15 from the membrane to promote medial division Molecular biology of the cell. 2020 Apr 14; 31 (9). mbcE20010026

2019

Mangione MC , Snider CE , Gould KL , The intrinsically disordered region of the cytokinetic F-BAR protein Cdc15 provides a unique essential function in maintenance of cytokinetic ring integrity Molecular biology of the cell. 2019 Sep 11; mbcE19060314
MacQuarrie CD , Mangione MC , Carroll R , James M , Gould KL , Sirotkin V , Adaptor protein Bbc1 regulates localization of Wsp1 and Vrp1 during endocytic actin patch assembly Journal of cell science. 2019 Aug 07;
Mangione MC , Gould KL , Molecular form and function of the cytokinetic ring Journal of cell science. 2019 Jun 17; 132 (12).
Willet AH , DeWitt AK , Beckley JR , Clifford DM , Gould KL , NDR Kinase Sid2 Drives Anillin-like Mid1 from the Membrane to Promote Cytokinesis and Medial Division Site Placement Current biology : CB. 2019 Mar 04;
Cullati SN , Gould KL , Spatiotemporal regulation of the Dma1-mediated mitotic checkpoint coordinates mitosis with cytokinesis Current genetics. 2019 Jan 2; (65). 663–8

2018

Neitzel LR , Broadus MR , Zhang N , Sawyer L , Wallace HA , Merkle JA , Jodoin JN , Sitaram P , Crispi EE , Rork W , Lee LA , Pan D , Gould KL , Page-McCaw A , Lee E , Characterization of a cdc14 null allele in Drosophila melanogaster Biology open. 2018 Jul 9; 7 (7).
Jones CM , Chen JS , Johnson AE , Elmore ZC , Cullati SN , Beckley JR , Gould KL , Relief of the Dma1-mediated checkpoint requires Dma1 autoubiquitination and dynamic localization Molecular biology of the cell. 2018 Jul 05; mbcE18040261
Snider CE , Willet AH , Brown HT , Gould KL , Analysis of the contribution of phosphoinositides to medial septation in fission yeast highlights the importance of PI(4,5)P for medial contractile ring anchoring Molecular biology of the cell. 2018 Jul 05; mbcE18030179
Willet AH , Bohnert KA , Gould KL , Cdk1-dependent phospho-inhibition of a formin-F-BAR interaction opposes cytokinetic contractile ring… Molecular biology of the cell. 2018 Jan 17;
Elmore ZC , Guillen RX , Gould KL , The kinase domain of CK1 enzymes contains the localization cue essential for compartmentalized signa… Molecular biology of the cell. 2018 May 9; mbcE18020129

2017

Snider CE , Willet AH , Chen JS , Arpağ G , Zanic M , Gould KL , Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis. The Journal of cell biology. 2017 Aug 7;
McDonald NA , Lind AL , Smith SE , Li R , Gould K , Nanoscale architecture of the Schizosaccharomyces pombe contractile ring. eLife. 2017 Sep 15; 6

2016

Chen JS , Beckley JR , Ren L , Feoktistova A , Jensen MA , Rhind N , Gould KL , Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregatio… Yeast (Chichester, England). 2016 May 11;
McDonald NA , Gould KL , Linking up at the BAR: oligomerization and F-BAR protein function. Cell cycle (Georgetown, Tex.). 2016 May 31;
Pyburn TM , Foegeding NJ , González-Rivera C , McDonald NA , Gould KL , Cover TL , Ohi MD , Structural organization of membrane-inserted hexamers formed by Helicobacter pylori VacA toxin. Molecular microbiology. 2016 Oct ; 102 (1). 22-36
Makarova M , Gu Y , Chen JS , Beckley JR , Gould KL , Oliferenko S , Temporal Regulation of Lipin Activity Diverged to Account for Differences in Mitotic Programs. Current biology : CB. 2016 Jan 25; 26 (2). 237-43
McDonald NA , Gould KL , Characterization of Cytokinetic F-BARs and Other Membrane-Binding Proteins. Methods in molecular biology (Clifton, N.J.). 2016 ; 1369 181-9
McDonald NA , Takizawa Y , Feoktistova A , Xu P , Ohi MD , Vander Kooi CW , Gould KL , The Tubulation Activity of a Fission Yeast F-BAR Protein Is Dispensable for Its Function in Cytokine… Cell reports. 2016 Jan 26; 14 (3). 534-46
Meyers FJ , Mathur A , Fuhrmann CN , O'Brien TC , Wefes I , Labosky PA , Duncan DS , August A , Feig A , Gould KL , Friedlander MJ , Schaffer CB , Van Wart A , Chalkley R , The origin and implementation of the Broadening Experiences in Scientific Training programs: an NIH … FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2016 Feb ; 30 (2). 507-14

2015

Beckley JR , Chen JS , Yang Y , Peng J , Gould KL , A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival. Molecular & cellular proteomics : MCP. 2015 Dec ; 14 (12). 3132-41
Chen JS , Beckley JR , McDonald NA , Ren L , Mangione M , Jang SJ , Elmore ZC , Rachfall N , Feoktistova A , Jones CM , Willet AH , Guillen R , Bitton DA , Bähler J , Jensen MA , Rhind N , Gould KL , Identification of new players in cell division, DNA damage response, and morphogenesis through const… G3 (Bethesda, Md.). 2015 Mar ; 5 (3). 361-70
Ren L , Willet AH , Roberts-Galbraith RH , McDonald NA , Feoktistova A , Chen JS , Huang H , Guillen R , Boone C , Sidhu SS , Beckley JR , Gould KL , The Cdc15 and Imp2 SH3 domains cooperatively scaffold a network of proteins that redundantly ensure … Molecular biology of the cell. 2015 Jan 15; 26 (2). 256-69
McDonald NA , Vander Kooi CW , Ohi MD , Gould KL , Oligomerization but Not Membrane Bending Underlies the Function of Certain F-BAR Proteins in Cell Mo… Developmental cell. 2015 Dec 21; 35 (6). 725-36
Willet AH , McDonald NA , Gould KL , Regulation of contractile ring formation and septation in Schizosaccharomyces pombe. Current opinion in microbiology. 2015 Dec ; 28 46-52
Willet AH , McDonald NA , Bohnert KA , Baird MA , Allen JR , Davidson MW , Gould KL , The F-BAR Cdc15 promotes contractile ring formation through the direct recruitment of the formin Cdc… The Journal of cell biology. 2015 Feb 16; 208 (4). 391-9

2014

Ma H , McLean JR , Gould KL , McCollum D , An efficient fluorescent protein-based multifunctional affinity purification approach in mammalian c… Methods in molecular biology (Clifton, N.J.). 2014 ; 1177 175-91
Rachfall N , Johnson AE , Mehta S , Chen JS , Gould KL , Cdk1 promotes cytokinesis in fission yeast through activation of the septation initiation network. Molecular biology of the cell. 2014 Aug 1; 25 (15). 2250-9
Collier SE , Voehler M , Peng D , Ohi R , Gould KL , Reiter NJ , Ohi MD , Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5. Biochemistry. 2014 Oct 21; 53 (41). 6439-51
Elmore ZC , Beckley JR , Chen JS , Gould KL , Histone H2B ubiquitination promotes the function of the anaphase-promoting complex/cyclosome in Schi… G3 (Bethesda, Md.). 2014 Aug ; 4 (8). 1529-38

2013

Johnson AE , Chen JS , Gould KL , CK1 is required for a mitotic checkpoint that delays cytokinesis. Current biology : CB. 2013 Oct 7; 23 (19). 1920-6
Chen JS , Broadus MR , McLean JR , Feoktistova A , Ren L , Gould KL , Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cd… Molecular & cellular proteomics : MCP. 2013 May ; 12 (5). 1074-86
Dephoure N , Gould KL , Gygi SP , Kellogg DR , Mapping and analysis of phosphorylation sites: a quick guide for cell biologists. Molecular biology of the cell. 2013 Mar ; 24 (5). 535-42
Gupta S , Mana-Capelli S , McLean JR , Chen CT , Ray S , Gould KL , McCollum D , Identification of SIN pathway targets reveals mechanisms of crosstalk between NDR kinase pathways. Current biology : CB. 2013 Feb 18; 23 (4). 333-8
Bohnert KA , Willet AH , Kovar DR , Gould KL , Formin-based control of the actin cytoskeleton during cytokinesis. Biochemical Society transactions. 2013 Dec ; 41 (6). 1750-4
Bohnert KA , Grzegorzewska AP , Willet AH , Vander Kooi CW , Kovar DR , Gould KL , SIN-dependent phosphoinhibition of formin multimerization controls fission yeast cytokinesis. Genes & development. 2013 Oct 1; 27 (19). 2164-77
Bajpai A , Feoktistova A , Chen JS , McCollum D , Sato M , Carazo-Salas RE , Gould KL , Csikász-Nagy A , Dynamics of SIN asymmetry establishment. PLoS computational biology. 2013 ; 9 (7). e1003147

2012

Pancaldi V , Saraç OS , Rallis C , McLean JR , Převorovský M , Gould K , Beyer A , Bähler J , Predicting the fission yeast protein interaction network. G3 (Bethesda, Md.). 2012 Apr ; 2 (4). 453-67
Ma H , McLean JR , Chao LF , Mana-Capelli S , Paramasivam M , Hagstrom KA , Gould KL , McCollum D , A highly efficient multifunctional tandem affinity purification approach applicable to diverse organ… Molecular & cellular proteomics : MCP. 2012 Aug ; 11 (8). 501-11
Lu LX , Domingo-Sananes MR , Huzarska M , Novak B , Gould KL , Multisite phosphoregulation of Cdc25 activity refines the mitotic entrance and exit switches. Proceedings of the National Academy of Sciences of the United States of America. 2012 Jun 19; 109 (25). 9899-904
Johnson NP , Gould KL , Integrating noninvasive absolute flow, coronary flow reserve, and ischemic thresholds into a compreh… JACC. Cardiovascular imaging. 2012 Apr ; 5 (4). 430-40
Feoktistova A , Morrell-Falvey J , Chen JS , Singh NS , Balasubramanian MK , Gould KL , The fission yeast septation initiation network (SIN) kinase, Sid2, is required for SIN asymmetry and… Molecular biology of the cell. 2012 May ; 23 (9). 1636-45
Bohnert KA , Gould KL , Cytokinesis-based constraints on polarized cell growth in fission yeast. PLoS genetics. 2012 ; 8 (10). e1003004
Mana-Capelli S , McLean JR , Chen CT , Gould KL , McCollum D , The kinesin-14 Klp2 is negatively regulated by the SIN for proper spindle elongation and telophase n… Molecular biology of the cell. 2012 Dec ; 23 (23). 4592-600
Clément A , Solnica-Krezel L , Gould KL , Functional redundancy between Cdc14 phosphatases in zebrafish ciliogenesis. Developmental dynamics : an official publication of the American Association of Anatomists. 2012 Dec ; 241 (12). 1911-21
Broadus MR , Gould KL , Multiple protein kinases influence the redistribution of fission yeast Clp1/Cdc14 phosphatase upon g… Molecular biology of the cell. 2012 Oct ; 23 (20). 4118-28
Wang Y , Li WZ , Johnson AE , Luo ZQ , Sun XL , Feoktistova A , McDonald WH , McLeod I , Yates JR , Gould KL , McCollum D , Jin QW , Dnt1 acts as a mitotic inhibitor of the spindle checkpoint protein dma1 in fission yeast. Molecular biology of the cell. 2012 Sep ; 23 (17). 3348-56
Johnson AE , McCollum D , Gould KL , Polar opposites: Fine-tuning cytokinesis through SIN asymmetry. Cytoskeleton (Hoboken, N.J.). 2012 Oct ; 69 (10). 686-99
Johnson AE , Collier SE , Ohi MD , Gould KL , Fission yeast Dma1 requires RING domain dimerization for its ubiquitin ligase activity and mitotic c… The Journal of biological chemistry. 2012 Jul 27; 287 (31). 25741-8

2011

Převorovský M , Atkinson SR , Ptáčková M , McLean JR , Gould K , Folk P , Půta F , Bähler J , N-termini of fungal CSL transcription factors are disordered, enriched in regulatory motifs and inhi… PloS one. 2011 ; 6 (8). e23650
Chen JS , Lu LX , Ohi MD , Creamer KM , English C , Partridge JF , Ohi R , Gould KL , Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation. The Journal of cell biology. 2011 Nov 14; 195 (4). 583-93
McLean JR , Kouranti I , Gould KL , Survey of the phosphorylation status of the Schizosaccharomyces pombe deubiquitinating enzyme (DUB) … Journal of proteome research. 2011 Mar 4; 10 (3). 1208-15
Clément A , Solnica-Krezel L , Gould KL , The Cdc14B phosphatase contributes to ciliogenesis in zebrafish. Development (Cambridge, England). 2011 Jan ; 138 (2). 291-302
Goettel JA , Liang D , Hilliard VC , Edelblum KL , Broadus MR , Gould KL , Hanks SK , Polk DB , KSR1 is a functional protein kinase capable of serine autophosphorylation and direct phosphorylation… Experimental cell research. 2011 Feb 15; 317 (4). 452-63
Johnson AE , Gould KL , Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase. The EMBO journal. 2011 Jan 19; 30 (2). 341-54
Singh NS , Shao N , McLean JR , Sevugan M , Ren L , Chew TG , Bimbo A , Sharma R , Tang X , Gould KL , Balasubramanian MK , SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in… Current biology : CB. 2011 Dec 6; 21 (23). 1968-78
Peckys DB , Mazur P , Gould KL , de Jonge N , Fully hydrated yeast cells imaged with electron microscopy. Biophysical journal. 2011 May 18; 100 (10). 2522-9
Ren L , McLean JR , Hazbun TR , Fields S , Vander Kooi C , Ohi MD , Gould KL , Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factor… PloS one. 2011 ; 6 (2). e16719
Almonacid M , Celton-Morizur S , Jakubowski JL , Dingli F , Loew D , Mayeux A , Chen JS , Gould KL , Clifford DM , Paoletti A , Temporal control of contractile ring assembly by Plo1 regulation of myosin II recruitment by Mid1/an… Current biology : CB. 2011 Mar 22; 21 (6). 473-9
Bohnert KA , Gould KL , On the cutting edge: post-translational modifications in cytokinesis. Trends in cell biology. 2011 May ; 21 (5). 283-92
McLean JR , Chaix D , Ohi MD , Gould KL , State of the APC/C: organization, function, and structure. Critical reviews in biochemistry and molecular biology. 2011 Apr ; 46 (2). 118-36

2010

Roberts-Galbraith RH , Gould KL , Setting the F-BAR: functions and regulation of the F-BAR protein family. Cell cycle (Georgetown, Tex.). 2010 Oct 15; 9 (20). 4091-7
Kouranti I , McLean JR , Feoktistova A , Liang P , Johnson AE , Roberts-Galbraith RH , Gould KL , A global census of fission yeast deubiquitinating enzyme localization and interaction networks revea… PLoS biology. 2010 ; 8 (9).
Roberts-Galbraith RH , Ohi MD , Ballif BA , Chen JS , McLeod I , McDonald WH , Gygi SP , Yates JR , Gould KL , Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding a… Molecular cell. 2010 Jul 9; 39 (1). 86-99
Vander Kooi CW , Ren L , Xu P , Ohi MD , Gould KL , Chazin WJ , The Prp19 WD40 domain contains a conserved protein interaction region essential for its function. Structure (London, England : 1993). 2010 May 12; 18 (5). 584-93
Papadopoulou K , Chen JS , Regulation of cell cycle-specific gene expression in fission yeast by the Cdc14p-like phosphatase Cl… Journal of cell science. 2010 Dec 15; 123 (Pt 24). 4374-81

2009

Bohnert KA , Chen JS , Clifford DM , Vander Kooi CW , Gould KL , A link between aurora kinase and Clp1/Cdc14 regulation uncovered by the identification of a fission … Molecular biology of the cell. 2009 Aug ; 20 (16). 3646-59
Roberts-Galbraith RH , Chen JS , Wang J , Gould KL , The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe con… The Journal of cell biology. 2009 Jan 12; 184 (1). 113-27

Liu Y , McDonald NA , Naegele SM , Gould KL , Wu JQ , The F-BAR Domain of Rga7 Relies on a Cooperative Mechanism of Membrane Binding with a Partner Protein during Fission Yeast Cytokinesis Cell reports. ; 26 (10). 2540-2548.e4