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Gould Lab

Gould Lab Publications

2024

Chen JS , Igarashi MG , Ren L , Hanna SM , Turner LA , McDonald NA , Beckley JR , Willet AH , Gould KL , The core spindle pole body scaffold Ppc89 links the pericentrin ortholog Pcp1 to the fission yeast spindle pole body via an evolutionarily conserved interface Molecular Biology of the Cell. 2024 Jul 10;
Turner LA , Willet AH , Gould KL , Characterization of temperature-sensitive alleles of Schizosaccharomyces pombe septation initiation network components microPublication Biology. 2024 Jun 25;
Willet AH , Ren L , Turner LA , Gould KL , Transient PP2A SIP complex localization to mitotic SPBs for SIN inhibition is mediated solely by the Csc1 FHA domain Molecular Biology of the Cell. 2024 Jun 14;
Fletcher AB , Turner LA , Ren L , Willet AH , Gould KL , Characterization of temperature-sensitive Schizosaccharomyces pombe mutants in the septation initiation network Spg1 GTPase microPublication Biology. 2024 Jun ;
Cullait SN , Akizuki K , Chen JS , Johnson JL , Yarin-Barir TM , Cantley LC , Gould KL , Substrate displacement of CK1 C-termini regulates kinase specificity Science Advances. 2024 May 10; 10 (19).
Tavafoghi B , Ren L , Gould KL , Willet AH , Generation and characterization of temperature-sensitive alleles encoding GPI anchored proteins Psu1 and Dfg502 in Schizosaccharomyces pombe microPublication Biology. 2024 Apr 2;
Iglesias-Ronero AB , Soto T , Flor-Parra I , Salas-Pino S , Ruiz-Romero G , Gould KL , Cansado J , Daga RR , MAPK-dependent control of mitotic progression in S. pombe BMC Biology. 2024 Mar 25; 71 (22).
Howard I.V. , Tavafoghi B , Igarashi M.G. , Ren L , Willet A.H. , Gould K.L. , Generation and characterization of temperature-sensitive alleles of the glucanosyltransferase Gas1 in Schizosaccharomyces pombe microPublication Biology. 2024 Mar 5;
Maury LL , Ren L , Hassan S , Bahler J , Gould KL , The Cdc14 phosphatase, Clp1, does not affect genome expression microPublication Biology. 2024 Feb ;

2023

Willet AH , Turner LA , Park JS , Ren L , Snider CE , Gould KL , Characterization of Pik1 function in fission yeast reveals its conserved role in lipid synthesis and not cytokinesis Journal of Cell Science. 2023 Oct 10;
Willet AH , Wos M , Igarashi MG , Ren L , Turner LA , Gould KL , Elevated levels of sphingolipid MIPC in the plasma membrane disrupt the coordination of cell growth with cell wall formation in fission yeast PLoS Genetics. 2023 Oct 4;
Lera-Ramirez M , Bahler J , Mata J , Rutherford K , Hoffman C , Lambert S , Oliferenko S , Martin SG , Gould KL , Du L , Sabatinos S , Forsburg SL , Nielsen O , Nurse P , Wood V , Revised fission yeast gene and allele nomenclature guidelines for machine readability Genetics. 2023 Sep 27;
Igrashi MG , Bhattacharjee R , Willet AH , Gould KL , Polarity kinases that phosphorylate F-BAR protein Cdc15 have unique localization patterns during cytokinesis and contributions to preventing tip septation in Schizosaccharomyces pombe microPublication Biology. 2023 Sep ;
Willet AH , Ren L , Turner LA , Gould KL , Isolation of mutant alleles of the U6 snRNA m6A methyltransferase Mtl16 and characterization of their genetic interactions with splicing mutants in Schizosaccharomyces pombe microPublication Biology. 2023 Aug 11;
Willet AH , Chen JS , Ren L , Gould KL , Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins Molecular Biology of the Cell. 2023 Aug 2;
Rossi AR , Bohnert KA , Gould KL , The fission yeast cytokinetic ring component Fic1 promotes septum formation Biology Open. 2023 May ; 5 (12).
Bhattacharjee R , Hall A , Mangione M , Igarashi M , Roberts-Galbraith R , Chen JS , Vavylonis D , Gould KL , Multiple polarity kinases inhibit phase separation of F-BAR protein Cdc15 and antagonize cytokinetic ring assembly in fission yeast eLife. 2023 Feb 7;

2022

Cullati SN , Chaikuad A , Chen JS , Grebel J , Tesmer L , Zhubi R , Navarrete-perea J , Guillen RX , Gygi SP , Hummer G , Dotsch V , Knapp S , Gould KL , Kinase domain autophosphorylation rewires the activity and substrate specificity of CK1 enzymes Molecular Cell. 2022 Mar 29;
Snider CE* , Bhattacharjee R* , Igarashi MG , Gould KL , Fission yeast paxillin contains two Cdc15 binding motifs for robust recruitment to the cytokinetic ring Molecular Biology of the Cell. 2022 Feb 2; 33 (4).

2021

Chen JS , Jones CM , Igarashi MG , Ren L , Johnson AE , Gould KL , Localization of the ubiquitin ligase Dma1 to the fission yeast contractile ring is modulated by phosphorylation FEBS Letters. 2021 Nov 2; (595). 2781–92
Willet AH , Igarashi MG , Chen JS , Bhattacharjee R , Ren L , Cullati SN , Elmore ZC , Roberts-Galbraith RH , Johnson AE , Beckley JR , Gould KL , Phosphorylation in the intrinsically disordered region of F-BAR protein Imp2 regulates its contractile ring recruitment Journal of Cell Science. 2021 Aug 17; 134 (16).
Mangione MC , Chen JS , Gould KL , Cdk1 phosphorylation of fission yeast paxillin inhibits its cytokinetic ring localization Molecular Biology of the Cell. 2021 Jun 16; 32 (17). 1534-44
Snider CE , Wan Mohamad Noor WNI , Nguyen NTH , Gould KL , Suetsugu S , The state of F-BAR domains as membrane-bound oligomeric platforms Trends in Cell Biology. 2021 Aug 20; 31 (8). 644-55

2020

Snider CE , Chandra M , McDonald N , Willet AH , Collier S , Ohi MD , Jackson LP , Gould KL , Opposite Surfaces of the Cdc15 F-BAR Domain Create a Membrane Platform That Coordinates Cytoskeletal and Signaling Components for Cytokinesis Cell Reports. 2020 Dec 22; 33 (12).
Snider CE , Willet AH , Brown HT , Chen JS , Evers JM , Gould KL , Fission yeast Opy1 is an endogenous PI(4,5)P sensor that binds the PI5-kinase Its3 Journal of cell science. 2020 Dec 3; 133 (23).
Bohnert KA , Rossi AM , Jin QW , Chen JS , Gould KL , Phosphoregulation of the Cytokinetic Protein Fic1 Contributes to Fission Yeast Growth Polarity Establishment Journal of cell science. 2020 Sep 2; 133 (18).
Guillen RX , Beckley JR , Chen JS , Gould KL , CRISPR-mediated gene targeting of CK1δ/ε leads to enhanced understanding of their role in endocytosis via phosphoregulation of GAPVD1 Scientific reports. 2020 Apr 22; 10 (1). 6797
Bhattacharjee R , Mangione MC , Wos M , Chen JS , Snider CE , Roberts-Galbraith RH , McDonald NA , Presti LL , Martin SG , Gould KL , DYRK kinase Pom1 drives F-BAR protein Cdc15 from the membrane to promote medial division Molecular biology of the cell. 2020 Apr 14; 31 (9). mbcE20010026

2019

Mangione MC , Snider CE , Gould KL , The intrinsically disordered region of the cytokinetic F-BAR protein Cdc15 provides a unique essential function in maintenance of cytokinetic ring integrity Molecular biology of the cell. 2019 Sep 11; mbcE19060314
MacQuarrie CD , Mangione MC , Carroll R , James M , Gould KL , Sirotkin V , Adaptor protein Bbc1 regulates localization of Wsp1 and Vrp1 during endocytic actin patch assembly Journal of cell science. 2019 Aug 07;
Mangione MC , Gould KL , Molecular form and function of the cytokinetic ring Journal of cell science. 2019 Jun 17; 132 (12).
Willet AH , DeWitt AK , Beckley JR , Clifford DM , Gould KL , NDR Kinase Sid2 Drives Anillin-like Mid1 from the Membrane to Promote Cytokinesis and Medial Division Site Placement Current biology : CB. 2019 Mar 04;
Cullati SN , Gould KL , Spatiotemporal regulation of the Dma1-mediated mitotic checkpoint coordinates mitosis with cytokinesis Current genetics. 2019 Jan 2; (65). 663–8

2018

Neitzel LR , Broadus MR , Zhang N , Sawyer L , Wallace HA , Merkle JA , Jodoin JN , Sitaram P , Crispi EE , Rork W , Lee LA , Pan D , Gould KL , Page-McCaw A , Lee E , Characterization of a cdc14 null allele in Drosophila melanogaster Biology open. 2018 Jul 9; 7 (7).
Jones CM , Chen JS , Johnson AE , Elmore ZC , Cullati SN , Beckley JR , Gould KL , Relief of the Dma1-mediated checkpoint requires Dma1 autoubiquitination and dynamic localization Molecular biology of the cell. 2018 Jul 05; mbcE18040261
Snider CE , Willet AH , Brown HT , Gould KL , Analysis of the contribution of phosphoinositides to medial septation in fission yeast highlights the importance of PI(4,5)P for medial contractile ring anchoring Molecular biology of the cell. 2018 Jul 05; mbcE18030179
Willet AH , Bohnert KA , Gould KL , Cdk1-dependent phospho-inhibition of a formin-F-BAR interaction opposes cytokinetic contractile ring… Molecular biology of the cell. 2018 Jan 17;
Elmore ZC , Guillen RX , Gould KL , The kinase domain of CK1 enzymes contains the localization cue essential for compartmentalized signa… Molecular biology of the cell. 2018 May 9; mbcE18020129

2017

Snider CE , Willet AH , Chen JS , ArpaÄź G , Zanic M , Gould KL , Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis. The Journal of cell biology. 2017 Aug 7;
McDonald NA , Lind AL , Smith SE , Li R , Gould K , Nanoscale architecture of the Schizosaccharomyces pombe contractile ring. eLife. 2017 Sep 15; 6

2016

Chen JS , Beckley JR , Ren L , Feoktistova A , Jensen MA , Rhind N , Gould KL , Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregatio… Yeast (Chichester, England). 2016 May 11;
McDonald NA , Gould KL , Linking up at the BAR: oligomerization and F-BAR protein function. Cell cycle (Georgetown, Tex.). 2016 May 31;
Pyburn TM , Foegeding NJ , González-Rivera C , McDonald NA , Gould KL , Cover TL , Ohi MD , Structural organization of membrane-inserted hexamers formed by Helicobacter pylori VacA toxin. Molecular microbiology. 2016 Oct ; 102 (1). 22-36
Makarova M , Gu Y , Chen JS , Beckley JR , Gould KL , Oliferenko S , Temporal Regulation of Lipin Activity Diverged to Account for Differences in Mitotic Programs. Current biology : CB. 2016 Jan 25; 26 (2). 237-43
McDonald NA , Gould KL , Characterization of Cytokinetic F-BARs and Other Membrane-Binding Proteins. Methods in molecular biology (Clifton, N.J.). 2016 ; 1369 181-9
McDonald NA , Takizawa Y , Feoktistova A , Xu P , Ohi MD , Vander Kooi CW , Gould KL , The Tubulation Activity of a Fission Yeast F-BAR Protein Is Dispensable for Its Function in Cytokine… Cell reports. 2016 Jan 26; 14 (3). 534-46
Meyers FJ , Mathur A , Fuhrmann CN , O'Brien TC , Wefes I , Labosky PA , Duncan DS , August A , Feig A , Gould KL , Friedlander MJ , Schaffer CB , Van Wart A , Chalkley R , The origin and implementation of the Broadening Experiences in Scientific Training programs: an NIH … FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2016 Feb ; 30 (2). 507-14

2015

Beckley JR , Chen JS , Yang Y , Peng J , Gould KL , A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival. Molecular & cellular proteomics : MCP. 2015 Dec ; 14 (12). 3132-41
Chen JS , Beckley JR , McDonald NA , Ren L , Mangione M , Jang SJ , Elmore ZC , Rachfall N , Feoktistova A , Jones CM , Willet AH , Guillen R , Bitton DA , Bähler J , Jensen MA , Rhind N , Gould KL , Identification of new players in cell division, DNA damage response, and morphogenesis through const… G3 (Bethesda, Md.). 2015 Mar ; 5 (3). 361-70
Ren L , Willet AH , Roberts-Galbraith RH , McDonald NA , Feoktistova A , Chen JS , Huang H , Guillen R , Boone C , Sidhu SS , Beckley JR , Gould KL , The Cdc15 and Imp2 SH3 domains cooperatively scaffold a network of proteins that redundantly ensure … Molecular biology of the cell. 2015 Jan 15; 26 (2). 256-69
McDonald NA , Vander Kooi CW , Ohi MD , Gould KL , Oligomerization but Not Membrane Bending Underlies the Function of Certain F-BAR Proteins in Cell Mo… Developmental cell. 2015 Dec 21; 35 (6). 725-36
Willet AH , McDonald NA , Gould KL , Regulation of contractile ring formation and septation in Schizosaccharomyces pombe. Current opinion in microbiology. 2015 Dec ; 28 46-52
Willet AH , McDonald NA , Bohnert KA , Baird MA , Allen JR , Davidson MW , Gould KL , The F-BAR Cdc15 promotes contractile ring formation through the direct recruitment of the formin Cdc… The Journal of cell biology. 2015 Feb 16; 208 (4). 391-9

2014

Ma H , McLean JR , Gould KL , McCollum D , An efficient fluorescent protein-based multifunctional affinity purification approach in mammalian c… Methods in molecular biology (Clifton, N.J.). 2014 ; 1177 175-91
Rachfall N , Johnson AE , Mehta S , Chen JS , Gould KL , Cdk1 promotes cytokinesis in fission yeast through activation of the septation initiation network. Molecular biology of the cell. 2014 Aug 1; 25 (15). 2250-9
Collier SE , Voehler M , Peng D , Ohi R , Gould KL , Reiter NJ , Ohi MD , Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5. Biochemistry. 2014 Oct 21; 53 (41). 6439-51
Elmore ZC , Beckley JR , Chen JS , Gould KL , Histone H2B ubiquitination promotes the function of the anaphase-promoting complex/cyclosome in Schi… G3 (Bethesda, Md.). 2014 Aug ; 4 (8). 1529-38

2013

Johnson AE , Chen JS , Gould KL , CK1 is required for a mitotic checkpoint that delays cytokinesis. Current biology : CB. 2013 Oct 7; 23 (19). 1920-6
Chen JS , Broadus MR , McLean JR , Feoktistova A , Ren L , Gould KL , Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cd… Molecular & cellular proteomics : MCP. 2013 May ; 12 (5). 1074-86
Dephoure N , Gould KL , Gygi SP , Kellogg DR , Mapping and analysis of phosphorylation sites: a quick guide for cell biologists. Molecular biology of the cell. 2013 Mar ; 24 (5). 535-42
Gupta S , Mana-Capelli S , McLean JR , Chen CT , Ray S , Gould KL , McCollum D , Identification of SIN pathway targets reveals mechanisms of crosstalk between NDR kinase pathways. Current biology : CB. 2013 Feb 18; 23 (4). 333-8
Bohnert KA , Willet AH , Kovar DR , Gould KL , Formin-based control of the actin cytoskeleton during cytokinesis. Biochemical Society transactions. 2013 Dec ; 41 (6). 1750-4
Bohnert KA , Grzegorzewska AP , Willet AH , Vander Kooi CW , Kovar DR , Gould KL , SIN-dependent phosphoinhibition of formin multimerization controls fission yeast cytokinesis. Genes & development. 2013 Oct 1; 27 (19). 2164-77
Bajpai A , Feoktistova A , Chen JS , McCollum D , Sato M , Carazo-Salas RE , Gould KL , Csikász-Nagy A , Dynamics of SIN asymmetry establishment. PLoS computational biology. 2013 ; 9 (7). e1003147

2012

Pancaldi V , Saraç OS , Rallis C , McLean JR , Převorovský M , Gould K , Beyer A , Bähler J , Predicting the fission yeast protein interaction network. G3 (Bethesda, Md.). 2012 Apr ; 2 (4). 453-67
Ma H , McLean JR , Chao LF , Mana-Capelli S , Paramasivam M , Hagstrom KA , Gould KL , McCollum D , A highly efficient multifunctional tandem affinity purification approach applicable to diverse organ… Molecular & cellular proteomics : MCP. 2012 Aug ; 11 (8). 501-11
Lu LX , Domingo-Sananes MR , Huzarska M , Novak B , Gould KL , Multisite phosphoregulation of Cdc25 activity refines the mitotic entrance and exit switches. Proceedings of the National Academy of Sciences of the United States of America. 2012 Jun 19; 109 (25). 9899-904
Johnson NP , Gould KL , Integrating noninvasive absolute flow, coronary flow reserve, and ischemic thresholds into a compreh… JACC. Cardiovascular imaging. 2012 Apr ; 5 (4). 430-40
Feoktistova A , Morrell-Falvey J , Chen JS , Singh NS , Balasubramanian MK , Gould KL , The fission yeast septation initiation network (SIN) kinase, Sid2, is required for SIN asymmetry and… Molecular biology of the cell. 2012 May ; 23 (9). 1636-45
Bohnert KA , Gould KL , Cytokinesis-based constraints on polarized cell growth in fission yeast. PLoS genetics. 2012 ; 8 (10). e1003004
Mana-Capelli S , McLean JR , Chen CT , Gould KL , McCollum D , The kinesin-14 Klp2 is negatively regulated by the SIN for proper spindle elongation and telophase n… Molecular biology of the cell. 2012 Dec ; 23 (23). 4592-600
Clément A , Solnica-Krezel L , Gould KL , Functional redundancy between Cdc14 phosphatases in zebrafish ciliogenesis. Developmental dynamics : an official publication of the American Association of Anatomists. 2012 Dec ; 241 (12). 1911-21
Broadus MR , Gould KL , Multiple protein kinases influence the redistribution of fission yeast Clp1/Cdc14 phosphatase upon g… Molecular biology of the cell. 2012 Oct ; 23 (20). 4118-28
Wang Y , Li WZ , Johnson AE , Luo ZQ , Sun XL , Feoktistova A , McDonald WH , McLeod I , Yates JR , Gould KL , McCollum D , Jin QW , Dnt1 acts as a mitotic inhibitor of the spindle checkpoint protein dma1 in fission yeast. Molecular biology of the cell. 2012 Sep ; 23 (17). 3348-56
Johnson AE , McCollum D , Gould KL , Polar opposites: Fine-tuning cytokinesis through SIN asymmetry. Cytoskeleton (Hoboken, N.J.). 2012 Oct ; 69 (10). 686-99
Johnson AE , Collier SE , Ohi MD , Gould KL , Fission yeast Dma1 requires RING domain dimerization for its ubiquitin ligase activity and mitotic c… The Journal of biological chemistry. 2012 Jul 27; 287 (31). 25741-8

2011

PĹ™evorovskĂ˝ M , Atkinson SR , Ptáčková M , McLean JR , Gould K , Folk P , PĹŻta F , Bähler J , N-termini of fungal CSL transcription factors are disordered, enriched in regulatory motifs and inhi… PloS one. 2011 ; 6 (8). e23650
Chen JS , Lu LX , Ohi MD , Creamer KM , English C , Partridge JF , Ohi R , Gould KL , Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation. The Journal of cell biology. 2011 Nov 14; 195 (4). 583-93
McLean JR , Kouranti I , Gould KL , Survey of the phosphorylation status of the Schizosaccharomyces pombe deubiquitinating enzyme (DUB) … Journal of proteome research. 2011 Mar 4; 10 (3). 1208-15
Clément A , Solnica-Krezel L , Gould KL , The Cdc14B phosphatase contributes to ciliogenesis in zebrafish. Development (Cambridge, England). 2011 Jan ; 138 (2). 291-302
Goettel JA , Liang D , Hilliard VC , Edelblum KL , Broadus MR , Gould KL , Hanks SK , Polk DB , KSR1 is a functional protein kinase capable of serine autophosphorylation and direct phosphorylation… Experimental cell research. 2011 Feb 15; 317 (4). 452-63
Johnson AE , Gould KL , Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase. The EMBO journal. 2011 Jan 19; 30 (2). 341-54
Singh NS , Shao N , McLean JR , Sevugan M , Ren L , Chew TG , Bimbo A , Sharma R , Tang X , Gould KL , Balasubramanian MK , SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in… Current biology : CB. 2011 Dec 6; 21 (23). 1968-78
Peckys DB , Mazur P , Gould KL , de Jonge N , Fully hydrated yeast cells imaged with electron microscopy. Biophysical journal. 2011 May 18; 100 (10). 2522-9
Ren L , McLean JR , Hazbun TR , Fields S , Vander Kooi C , Ohi MD , Gould KL , Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factor… PloS one. 2011 ; 6 (2). e16719
Almonacid M , Celton-Morizur S , Jakubowski JL , Dingli F , Loew D , Mayeux A , Chen JS , Gould KL , Clifford DM , Paoletti A , Temporal control of contractile ring assembly by Plo1 regulation of myosin II recruitment by Mid1/an… Current biology : CB. 2011 Mar 22; 21 (6). 473-9
Bohnert KA , Gould KL , On the cutting edge: post-translational modifications in cytokinesis. Trends in cell biology. 2011 May ; 21 (5). 283-92
McLean JR , Chaix D , Ohi MD , Gould KL , State of the APC/C: organization, function, and structure. Critical reviews in biochemistry and molecular biology. 2011 Apr ; 46 (2). 118-36

2010

Roberts-Galbraith RH , Gould KL , Setting the F-BAR: functions and regulation of the F-BAR protein family. Cell cycle (Georgetown, Tex.). 2010 Oct 15; 9 (20). 4091-7
Kouranti I , McLean JR , Feoktistova A , Liang P , Johnson AE , Roberts-Galbraith RH , Gould KL , A global census of fission yeast deubiquitinating enzyme localization and interaction networks revea… PLoS biology. 2010 ; 8 (9).
Roberts-Galbraith RH , Ohi MD , Ballif BA , Chen JS , McLeod I , McDonald WH , Gygi SP , Yates JR , Gould KL , Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding a… Molecular cell. 2010 Jul 9; 39 (1). 86-99
Vander Kooi CW , Ren L , Xu P , Ohi MD , Gould KL , Chazin WJ , The Prp19 WD40 domain contains a conserved protein interaction region essential for its function. Structure (London, England : 1993). 2010 May 12; 18 (5). 584-93
Papadopoulou K , Chen JS , Regulation of cell cycle-specific gene expression in fission yeast by the Cdc14p-like phosphatase Cl… Journal of cell science. 2010 Dec 15; 123 (Pt 24). 4374-81

2009

Bohnert KA , Chen JS , Clifford DM , Vander Kooi CW , Gould KL , A link between aurora kinase and Clp1/Cdc14 regulation uncovered by the identification of a fission … Molecular biology of the cell. 2009 Aug ; 20 (16). 3646-59
Roberts-Galbraith RH , Chen JS , Wang J , Gould KL , The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe con… The Journal of cell biology. 2009 Jan 12; 184 (1). 113-27

Liu Y , McDonald NA , Naegele SM , Gould KL , Wu JQ , The F-BAR Domain of Rga7 Relies on a Cooperative Mechanism of Membrane Binding with a Partner Protein during Fission Yeast Cytokinesis Cell reports. ; 26 (10). 2540-2548.e4