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Characterization of Cytokinetic F-BARs and Other Membrane-Binding Proteins.


McDonald NANathan A , Gould KL Kathleen L . Methods in molecular biology (Clifton, N.J.). 2016 ; 1369(). 181-9


Multiple membrane-binding proteins are key players in cytokinesis in yeast and other organisms. In vivo techniques for analyzing protein-membrane interactions are currently limited. In vitro assays allow characterization of the biochemical properties of these proteins to build a mechanistic understanding of protein-membrane interactions during cytokinesis. Here, we describe two in vitro assays to characterize FCH-Bin/Amphyphysin/RVS (F-BAR) domains and other protein’s interactions with membranes: liposome co-pelleting and giant unilamellar vesicle fluorescent binding.