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Identification of new players in cell division, DNA damage response, and morphogenesis through construction of Schizosaccharomyces pombe deletion strains.


Chen JSJun-Song , Beckley JR Janel R , McDonald NA Nathan A , Ren L Liping , Mangione M MariaSanta , Jang SJ Sylvia J , Elmore ZC Zachary C , Rachfall N Nicole , Feoktistova A Anna , Jones CM Christine M , Willet AH Alaina H , Guillen R Rodrigo , Bitton DA Danny A , Bähler J Jürg , Jensen MA Michael A , Rhind N Nick , Gould KL Kathleen L . G3 (Bethesda, Md.). 2015 3 ; 5(3). 361-70


Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of different stress conditions. We identified new genes involved in DNA metabolism, completion of the cell cycle, and morphogenesis. This subset of nonessential gene deletions will add to the toolkits available for the study of biological processes in S. pombe.