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Mapping and analysis of phosphorylation sites: a quick guide for cell biologists.


Dephoure NNoah , Gould KL Kathleen L , Gygi SP Steven P , Kellogg DR Douglas R . Molecular biology of the cell. 2013 3 ; 24(5). 535-42


A mechanistic understanding of signaling networks requires identification and analysis of phosphorylation sites. Mass spectrometry offers a rapid and highly sensitive approach to mapping phosphorylation sites. However, mass spectrometry has significant limitations that must be considered when planning to carry out phosphorylation-site mapping. Here we provide an overview of key information that should be taken into consideration before beginning phosphorylation-site analysis, as well as a step-by-step guide for carrying out successful experiments.