>

Histology

Location: MRBIII U3202

cryo

Leica CM1900

 

Reservations required in  VU iLab website

Overview

If you want to use a glass anti-roll plate, you are required to purchase one of these from the company. It is a “non-file” item number 0000707-06. Do not let them sell you the more expensive anti-roll plate which contains the metal arm which holds the glass. It is not necessary.

The CDB Equipment Core retains five 25 mm and five 38 mm round specimen holders. These can be signed out from the U3202 equipment room for use. However, the holders must be cleaned and returned to the equipment room after you finish sectioning. If your lab would like the convenience of having your own holders, ordering information is below.

Consumables

 

Tissue-Tek® Cryomold®:

#4728 Standard round mold (VWR Cat# 89199-518) – case of 1200
#4730 Biopsy round mold (VWR Cat# 89199-520) – case of 1200

#4566 Intermediate square mold 15 mm x 15 mm (VWR Cat# 25608-924) – pack of 100 or case of 1200
#4730 Standard rectangle mold 25 mm x 20 mm (VWR Cat# 25608-916) – pack of 100 or case of 1200

*Note: The largest rectangular cryomold may work best with the rake. This is due to the way the tissue needs to be mounted for optimal sectioning. If you are unsure of the size, purchase a pack of 100 molds to get you started.

Round Specimen Holders:

#5807 25 mm (VWR Cat# 89199-522) – six holders
#5808 38 mm (VWR Cat# 89199-524) – six holders
#5809 55 mm (VWR Cat# 89199-526) – six holders

Tissue-Tek® O.C.T. Compound:

#4583 (VWR Cat# 25608-930) – 4 oz bottle

Tissue-Tek® Accu-Edge®
Disposable Blades:

#4685 High-Profile (VWR Cat# 25608-961) – pack of 50
#4689 Low-Profile (VWR Cat# 25608-964) – pack of 50

Technical Specifications

 
Section Thickness Setting1 to 60um
Maximum Specimen Size55mm
Horizontal Specimen Feed25mm
Vertical Stroke59mm
Specimen RetractionOptional
Specimen OrientationOptional
TrimmingVia motorized coarse feed
Motorized Coarse Feed0.8 mm/sec
Specimen Cooling Temperature-10 °C down to -50 °C in 1 K increments
DefrostingManual defrost cycle
Cryochamber Cooling Range0 °C down to -35 °C
Cooling time down to -35 °C4 h
DefrostingProgrammable
1 Automatic defrost cycle/24 h
Duration9 min manual defrost cycle
Temperature of Quick Freeze ShelfDown to – 45 °C
(depending on the chamber temperature)
Power Draw1800 VA
Dimensions (W x H x D)890 x 1200 x 730 mm
Weight (including microtome)170 kg, 375 lbs