Location: MRBIII U3202
Leica CM1900
Reservations required in VU iLab website
Overview
If you want to use a glass anti-roll plate, you are required to purchase one of these from the company. It is a “non-file” item number 0000707-06. Do not let them sell you the more expensive anti-roll plate which contains the metal arm which holds the glass. It is not necessary.
The CDB Equipment Core retains five 25 mm and five 38 mm round specimen holders. These can be signed out from the U3202 equipment room for use. However, the holders must be cleaned and returned to the equipment room after you finish sectioning. If your lab would like the convenience of having your own holders, ordering information is below.
Consumables
Tissue-Tek® Cryomold®:
#4728 Standard round mold (VWR Cat# 89199-518) – case of 1200
#4730 Biopsy round mold (VWR Cat# 89199-520) – case of 1200
#4566 Intermediate square mold 15 mm x 15 mm (VWR Cat# 25608-924) – pack of 100 or case of 1200
#4730 Standard rectangle mold 25 mm x 20 mm (VWR Cat# 25608-916) – pack of 100 or case of 1200
*Note: The largest rectangular cryomold may work best with the rake. This is due to the way the tissue needs to be mounted for optimal sectioning. If you are unsure of the size, purchase a pack of 100 molds to get you started.
Round Specimen Holders:
#5807 25 mm (VWR Cat# 89199-522) – six holders
#5808 38 mm (VWR Cat# 89199-524) – six holders
#5809 55 mm (VWR Cat# 89199-526) – six holders
Tissue-Tek® O.C.T. Compound:
#4583 (VWR Cat# 25608-930) – 4 oz bottle
Tissue-Tek® Accu-Edge®
Disposable Blades:
#4685 High-Profile (VWR Cat# 25608-961) – pack of 50
#4689 Low-Profile (VWR Cat# 25608-964) – pack of 50
Technical Specifications
| Section Thickness Setting | 1 to 60um |
| Maximum Specimen Size | 55mm |
| Horizontal Specimen Feed | 25mm |
| Vertical Stroke | 59mm |
| Specimen Retraction | Optional |
| Specimen Orientation | Optional |
| Trimming | Via motorized coarse feed |
| Motorized Coarse Feed | 0.8 mm/sec |
| Specimen Cooling Temperature | -10 °C down to -50 °C in 1 K increments |
| Defrosting | Manual defrost cycle |
| Cryochamber Cooling Range | 0 °C down to -35 °C |
| Cooling time down to -35 °C | 4 h |
| Defrosting | Programmable 1 Automatic defrost cycle/24 h |
| Duration | 9 min manual defrost cycle |
| Temperature of Quick Freeze Shelf | Down to – 45 °C (depending on the chamber temperature) |
| Power Draw | 1800 VA |
| Dimensions (W x H x D) | 890 x 1200 x 730 mm |
| Weight (including microtome) | 170 kg, 375 lbs |