David C. Koomen
Graduate Student, The McLean Group
University of Tennessee – B.S. in Microbiology
University of South Florida – M.S. in Biotechnology
Background:
David was born and raised in Nashville, TN and attended the University of Tennessee in Knoxville, receiving his Bachelor’s in Microbiology. After graduating he took a job as a research assistant at H. Lee Moffitt Cancer Center in Tampa, FL studying drug resistance in multiple myeloma under the supervision of Ken H. Shain, MD, PhD. While working he continued his studies at the University of South Florida’s Morsani College of Medicine, receiving his Master’s in Biotechnology before coming to Vanderbilt to pursue a doctorate in Chemistry in the laboratory of Prof. John A. McLean.
David joined the McLean lab in January of 2021 and has mainly focused his research on incorporating ion mobility as an additional separation strategy into conventional untargeted liquid chromatography-mass spectrometry workflows for high-confidence annotation in metabolomics, lipidomics and exposomics. He has worked on several projects, including characterization of phase II sulfate and glucuronide steroid isomers in urine, deployment of rapid annotation strategies for identifying unknown metabolites of anabolic androgenic steroids, and development of data processing pipelines for high resolution demultiplexing drift tube ion mobility in untargeted lipidomic analyses. Currently, he is working on characterizing and quantifying perfluoroalkyl substances (PFAS) in human serum for non-targeted exposomic analysis and developing methods for investigating isomeric PFAS separation on different HRIM platforms.
Awards:
Mitchum Warren Fellowship, Vanderbilt University (August 2020 – June 2021)
Graduate Student Award, American Society for Mass Spectrometry (June 2023)
Publications:
David C. Koomen, Katrina L. Leaptrot, Jody C. May, Bailey S. Rose, Kyle E. Lira, Julia A. Raziel, Andrew D. Pumford, Gustavo de A. Cavalcanti, Monica C. Padilha, Henrique M. G. Pereira, John A. McLean. “Rapid Annotation Strategy for in vivo Phase II Metabolites of Anabolic Androgenic Steroids using Liquid Chromatography-Ion Mobility-Mass Spectrometry.” Clin Chem. 2024. Manuscript in preparation.
David C. Koomen, Jody C. May, Alexander J. Mansueto, Todd R. Graham, John A. McLean. “An Untargeted Lipidomics Workflow Incorporating High Resolution Demultiplexing (HRdm) Drift Tube Ion Mobility-Mass Spectrometry.” JASMS. 2024. Manuscript in review.
David C. Koomen, Jody C. May, John A. McLean. “Insights and prospects for ion mobility-mass spectrometry in clinical chemistry.” Expert Rev Proteomics. 2022; 19(1):17-31. doi: 10.1080/14789450.2022.2026218. PMID: 34986717; PMCID: PMC8881341.
Don E. Davis, Jr., Katrina L. Leaptrot, David C. Koomen, Jody C. May, Gustavo de A. Cavalcanti, Monica C. Padilha, Henrique M.G. Pereira, John A. McLean. “Multidimensional Separations of Intact Phase II Steroid Metabolites Utilizing LC-Ion Mobility-MS.”Analytical Chemistry. 2021; 93(31):10990-10998. doi: 10.1021/acs.analchem.1c02163. PMID: 34319704; PMCID: PMC9288154.
David C. Koomen, Mark B. Meads, Dario M. Magaletti, Joy D. Guingab-Cagmat, Paula S. Oliveira, Bin Fang, Min Liu, Eric A. Welsh, Laurel E. Meke, Zhijie Jiang, Oliver A. Hampton, Alexandre Tungesvik, Gabriel DeAvila, Raghunandan Reddy Alugubelli, Taiga Nishihori, Ariosto S. Silva, Steven A. Eschrich, Timothy J. Garrett, John M. Koomen, Kenneth H. Shain. “Metabolic Changes are Associated with Melphalan Resistance in Multiple Myeloma.“ J Proteome Res. 2021; 20(6):3134-3149. doi: 10.1021/acs.jproteome.1c00022. PMID: 34014671.
David C. Koomen,* Joy D. Guingab-Cagmat,* Paula S. Oliveira,* Bin Fang, Min Liu, Eric A. Welsh, Mark B. Meads, Tuan Nguyen, Laurel E. Meke, Steven A. Eschrich, Kenneth H. Shain, Timothy J. Garrett, John M. Koomen. “Proteometabolomics of Melphalan Resistance in Multiple Myeloma.” Methods in Molecular Biology. 2019; 1996:273-296. doi: 10.1007/978-1-4939-9488-5_21. PMID: 31127562; PMCID: PMC7771550.