Isolation of specific neurons from C. elegans larvae for gene expression profiling.
AUTHORS
Spencer
WCW Clay ,
McWhirter
R Rebecca ,
Miller
T Tyne ,
Strasbourger
P Pnina ,
Thompson
O Owen ,
Hillier
LW LaDeana W ,
Waterston
RH Robert H ,
Miller
DM David M .
PloS One. 2014 ; 9(11).
e112102
- PMID: 25372608[PubMed].
- PMCID: PMC4221280.
ABSTRACT
The simple and well-described structure of the C. elegans nervous system offers an unprecedented opportunity to identify the genetic programs that define the connectivity and function of individual neurons and their circuits. A correspondingly precise gene expression map of C. elegans neurons would facilitate the application of genetic methods toward this goal. Here we describe a powerful new approach, SeqCeL (RNA-Seq of C. elegans cells) for producing gene expression profiles of specific larval C. elegans neurons.