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Isolation of specific neurons from C. elegans larvae for gene expression profiling.


Spencer WCW Clay , McWhirter R Rebecca , Miller T Tyne , Strasbourger P Pnina , Thompson O Owen , Hillier LW LaDeana W , Waterston RH Robert H , Miller DM David M . PloS One. 2014 ; 9(11). e112102


The simple and well-described structure of the C. elegans nervous system offers an unprecedented opportunity to identify the genetic programs that define the connectivity and function of individual neurons and their circuits. A correspondingly precise gene expression map of C. elegans neurons would facilitate the application of genetic methods toward this goal. Here we describe a powerful new approach, SeqCeL (RNA-Seq of C. elegans cells) for producing gene expression profiles of specific larval C. elegans neurons.