Exosome Analysis for Biomarker Discovery

The Schey lab has begun a collaboration with Dr. David Hachey (Pharmacology)  and Dr. Matt Luther (Clinical Pharmacology) to examine the proteome and transcriptome of small lipid microvesicles (exosomes) secreted from cells and found in multiple body fluids. The driving hypothesis is that the exosomes contain signatures of disease that can be deciphered by proteomics and transcriptomics technologies.

 

Electron micrograph of isolated urinary exosomes.

 

Related References

Qi Y, Wang X, Rose KL, McDonald WH, Zhang B, Schey KL, Luther JM. Activation of the Endogenous Renin-Angiotensin-Aldosterone System or Aldosterone Administration Acutely Alter the Urinary Exosomal Proteome in Humans. J. Amer. Soc. Nephrol. 27:646, 2016.

Schey KL, Luther JM, Rose KL. Proteomics Characterization of Exosome Cargo. Methods. 87:75-82, 2015.

Wang Z, Hill S, Luther JM, Hachey DL, Schey KL. Proteomic Analysis of Urine Exosomes by Multidimensional Protein Identification Technology (MudPIT). Proteomics 12:329-338, 2012.

Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL. Protein Profile of Exosomes from Trabecular Meshwork Cells, J. Proteomics 74:796-804, 2011.