We are interested in the molecular machinery that drives and maintains apical/basal polarity and spindle orientation, and use several different systems to study this – MDCK cells grown as acini in 3D culture; and mouse mammary glands, which provide a wonderful model of branching morphogenesis. We are using the new CRISPR/Cas9 gene editing system to generate knockout cell lines and mice, to facilitate the study of the cell polarity machinery. We are also interested in how defects in cell polarity can promote breast cancer.