Crystallization and preliminary crystallographic studies of Gi alpha 1 and mutants of Gi alpha 1 in the GTP and GDP-bound states.
AUTHORS
Coleman
DED E ,
Lee
E E ,
Mixon
MB M B ,
Linder
ME M E ,
Berghuis
AM A M ,
Gilman
AG A G ,
Sprang
SR S R .
Journal of Molecular Biology. 1994 5 13; 238(4).
630-4
- PMID: 8176751[PubMed].
ABSTRACT
Several different crystal forms of Gi alpha 1 have been grown and analyzed. Crystals of native protein containing bound GTP gamma S belong to space group P3(1)2(1) or P3(2)2(1) with cell dimensions a,b = 80.6 A and c = 106.3 A and diffract to a resolution of 1.9 A using synchrotron radiation. Crystals of native protein containing bound GDP belong to space group I4 with cell dimensions a,b = 121.3 A, and c = 67.7 A and diffract to 3.0 A. Data sets from crystals grown using mutant proteins have also been obtained and characterized.