Pac-Van Facilities
- Facilities-Resources-Equipment Summary for your NiH Grant
- Pac-Van Facilities
- Reserve Instrument Time
- Singh P K , Cecchini G , Nakagawa T , Iverson T M , CryoEM structure of a post-assembly MS-ring reveals plasticity in stoichiometry and conformation PloS one. 2023 May 19; 18 (5). e0285343
- Singh P K, Sharma P, Afanzar O, Goldfarb M H , Maklashina E, Eisenbach M, Cecchini G, Iverson T M, CryoEM structures reveal how the bacterial flagellum rotates and switches direction Nature Microbiology. 2024 Apr 17
Mosquito LCP Robot
Crystallization trial experiments can be performed quickly and efficiently in 96 well plates, using the Mosquito, a nanoliter dispensing high-throughput robot.
- Uses disposable tips on a reel to dispense the sample from an 8-slot strip and a 96-well tray to a single drop in a sitting or hanging drop setup.
- Offers the ability to use a small amount of protein/DNA in high-throughput broad crystallization screening with no cross-contamination.
- Ratio of sample: solution, drop size, and number of samples per tray can be altered for each screen.
- Efficient setup time: 2 min per 96-well plate
- Minimal sample consumption: 10-50 µL of protein sample per 96-well plate
Videos: Overview and How The Mosquito Works.
Rock Imager
This is a robust, easy-to-use, automated imaging system for protein crystallization.
- Incubates and captures superior quality images of up to 1000 micro-titer plates on a user-defined schedule.
- Supports 500 LCP plates in addition to the above mentioned 1000 micro-titer plates.
- Collected images can be viewed remotely from any computer with a web browser.
- UV imaging uses the fluorescence of tryptophan under ultraviolet light to help determine if a particular crystal is protein, and aids in finding hidden crystals not visible with bright-field imaging.
