TAP Tag Information

Our S. pombe tandem affinity purification (TAP) protocol was adapted from the Seraphin lab S. cerevisiae protocol (see reference and website below). Click here for the pdf version of our protocol. 

Rigaut, G., Shevchenko, A., Rutz, B., Wilm, M., Mann, M., and Seraphin, B. 1999. A generic protein purification method for protein complex characterization and proteome exploration. Nature Biotechnology 17: 1030-1032. 

 

Our TAP Tag vector schematics and sequences:

2X PA CTAP in pFA6a | Schematic | Sequence | HTML | PDF
4X PA CTAP in pFA6a | Schematic | Sequence | HTML | PDF
NTAP Cassette | Schematic | Sequence | HTML | PDF
NTAP TAG | Sequence | HTML | PDF
pREP-NTAP Vector | Schematic | HTML | PDF
3HA 2XPA CTAP pFS209 (from N. Rhind)