Identification of new players in cell division, DNA damage response, and morphogenesis through construction of Schizosaccharomyces pombe deletion strains.
AUTHORS
Chen
JSJun-Song ,
Beckley
JR Janel R ,
McDonald
NA Nathan A ,
Ren
L Liping ,
Mangione
M MariaSanta ,
Jang
SJ Sylvia J ,
Elmore
ZC Zachary C ,
Rachfall
N Nicole ,
Feoktistova
A Anna ,
Jones
CM Christine M ,
Willet
AH Alaina H ,
Guillen
R Rodrigo ,
Bitton
DA Danny A ,
Bähler
J Jürg ,
Jensen
MA Michael A ,
Rhind
N Nick ,
Gould
KL Kathleen L .
G3 (Bethesda, Md.). 2015 3 ; 5(3).
361-70
- PMID: 25552606[PubMed].
- PMCID: PMC4349090.
ABSTRACT
Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of different stress conditions. We identified new genes involved in DNA metabolism, completion of the cell cycle, and morphogenesis. This subset of nonessential gene deletions will add to the toolkits available for the study of biological processes in S. pombe.