Mapping and analysis of phosphorylation sites: a quick guide for cell biologists.
AUTHORS
Dephoure
NNoah ,
Gould
KL Kathleen L ,
Gygi
SP Steven P ,
Kellogg
DR Douglas R .
Molecular biology of the cell. 2013 3 ; 24(5).
535-42
- PMID: 23447708[PubMed].
- PMCID: PMC3583658.
ABSTRACT
A mechanistic understanding of signaling networks requires identification and analysis of phosphorylation sites. Mass spectrometry offers a rapid and highly sensitive approach to mapping phosphorylation sites. However, mass spectrometry has significant limitations that must be considered when planning to carry out phosphorylation-site mapping. Here we provide an overview of key information that should be taken into consideration before beginning phosphorylation-site analysis, as well as a step-by-step guide for carrying out successful experiments.