Papers online at MBoC
Two papers are now online. Both projects were collaborations between multiple Gould lab members and we are excited that these results are now available.
Post-doc Alaina Willet, grad student Chloe Snider, and undergraduate student Hannah Brown collaborated to determine if any additional lipid species are important for cell division in S. pombe, building on our previous work highlighting the importance of PI(4,5)P2. Experiments in this paper employ two new PIP sensors for PI4P and PI(3,4,5)P2. To read more, visit doi.org/10.1091/mbc.E18-03-0179
Grad student Christine Jones and senior scientist Junsong Chen collaborated to discover how a cytokinesis checkpoint is resolved after resolution of spindle stress. By combining microscopy, biochemistry, and mass spectrometry with meticulous analysis, they determined that the ubiquitin ligase Dma1 auto-ubiquitinates to self-regulate its localization and access to substrates. To read more, visit doi.org/10.1091/mbc.E18-04-0261
Cartoon showing dynamic localization of Dma1 throughout mitosis and cytokinesis. In metaphase when the mitotic checkpoint is activated, Dma1 binds and ubiquitinates Sid4. Our data indicate that in early anaphase, Dma1 auto-ubiquitinates and leaves the SPB allowing maximal SIN activation and cytokinesis.
Image from doi.org/10.1091/mbc.E18-04-0261
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