Research

We have found that oncogenic mutation in the pancreas leads to genesis of a number of different secretory cell types, including tuft cells (Pink, COX1; Green, POU2F3; White, ac-a-tubulin; Blue, nuclei).

We use a number of different imaging strategies to study these populations, including immunofluorescence (Green, ac-a-tubulin; Blue, nuclei).

Scanning electron microscopy allows us to investigate the cell surface. Tuft cells are known for their long, blunt microvilli and structure often provides clues to function.

We use small cell number RNA-sequencing strategies to identify potential mechanisms by which rare cell types impact tumor formation and progression.

We use lineage-tracing strategies to identify where these rare secretory cell come from and to explore the clonal evolution of metaplasic ducts in disease progression (Red, Actin; Green, clones).

Pancreatitis is a major risk factor for pancreatic cancer. We have identified formation of these rare cell populations in the injured pancreas and are currently investigating their role in injury, healing, and pancreatic regeneration (Red, Actin; Green, YFP).

Combining 3View serial block face scanning electron microscopy and imaging software, we generate 3D images of secretory cell types, like tuft cells, to understand their subcellular structures and their interactions with stromal cells and other epithelial cells.

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These techniques have allowed us to discover previously un-described subcellular organelles in neoplastic tuft cells, including nucleus-associated lipid droplets.

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