Pankaj Sharma, Ph.D.

Pankaj Sharma, Ph.D.

Research Assistant Professor, Department of Pharmacology

: pankaj.sharma@vanderbilt.edu
: (615) 322-8721 Lab
: 464 Robinson Research Building
2200 Pierce Ave
Nashville, Tennessee - 37232

Background

Postdoc, Vanderbilt University

Ph.D. Institute of Microbial Technology, Chandigarh, India.

M.S. Guru Nanak Dev University, Amritsar, India.

Awards

AHA Postdoctoral Fellowship 2019-2020

Publications from the lab

CryoEM structures reveal how the bacterial flagellum rotates and switches direction. Singh PK, Sharma P, Afanzar O, Goldfarb MH, Maklashina E, Eisenbach M, Cecchini G, Iverson TM.Nat Microbiol. 2024 May;9(5):1271-1281. doi: 10.1038/s41564-024-01674-1. Epub 2024 Apr 17.PMID: 38632342

Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners. Sharma P, Maklashina E, Voehler M, Balintova S, Dvorakova S, Kraus M, Hadrava Vanova K, Nahacka Z, Zobalova R, Boukalova S, Cunatova K, Mracek T, Ghayee HK, Pacak K, Rohlena J, Neuzil J, Cecchini G, Iverson TM.Nat Commun. 2024 Jan 11;15(1):473. doi: 10.1038/s41467-023-44563-7.PMID: 38212624

EvdS6 is a bifunctional decarboxylase from the everninomicin gene cluster. Dulin CC, Sharma P, Frigo L, Voehler MW, Iverson TM, Bachmann BO.J Biol Chem. 2023 Jul;299(7):104893. doi: 10.1016/j.jbc.2023.104893. Epub 2023 Jun 5.PMID: 37286037

Base excision repair of the N-(2-deoxy-d-erythro-pentofuranosyl)-urea lesion by the hNEIL1 glycosylase. Tomar R, Minko IG, Sharma P, Kellum AH, Lei L, Harp JM, Iverson TM, Lloyd RS, Egli M, Stone MP.Nucleic Acids Res. 2023 May 8;51(8):3754-3769. doi: 10.1093/nar/gkad164.PMID: 37014002 Free PMC article.

The Two Non-Visual Arrestins Engage ERK2 Differently. Perry-Hauser NA, Hopkins JB, Zhuo Y, Zheng C, Perez I, Schultz KM, Vishnivetskiy SA, Kaya AI, Sharma P, Dalby KN, Chung KY, Klug CS, Gurevich VV, Iverson TM.J Mol Biol. 2022 Apr 15;434(7):167465. doi: 10.1016/j.jmb.2022.167465. Epub 2022 Jan 22.PMID: 35077767

An Eight Amino Acid Segment Controls Oligomerization and Preferred Conformation of the two Non-visual Arrestins. Chen Q, Zhuo Y, Sharma P, Perez I, Francis DJ, Chakravarthy S, Vishnivetskiy SA, Berndt S, Hanson SM, Zhan X, Brooks EK, Altenbach C, Hubbell WL, Klug CS, Iverson TM, Gurevich VV.J Mol Biol. 2021 Feb 19;433(4):166790. doi: 10.1016/j.jmb.2020.166790. Epub 2020 Dec 31.PMID: 33387531

The roles of SDHAF2 and dicarboxylate in covalent flavinylation of SDHA, the human complex II flavoprotein.

Sharma P, Maklashina E, Cecchini G, Iverson TM.Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23548-23556. doi: 10.1073/pnas.2007391117. Epub 2020 Sep 4.PMID: 32887801 Free PMC article.

Tandem sialoglycan-binding modules in a Streptococcus sanguinis serine-rich repeat adhesin create target dependent avidity effects. Stubbs HE, Bensing BA, Yamakawa I, Sharma P, Yu H, Chen X, Sullam PM, Iverson TM.J Biol Chem. 2020 Oct 23;295(43):14737-14749. doi: 10.1074/jbc.RA120.014177. Epub 2020 Aug 20.PMID: 32820052

Maturation of the respiratory complex II flavoprotein. Sharma P, Maklashina E, Cecchini G, Iverson TM.Curr Opin Struct Biol. 2019 Dec;59:38-46. doi: 10.1016/j.sbi.2019.01.027. Epub 2019 Mar 7.PMID: 30851631 Review.

Crystal structure of an assembly intermediate of respiratory Complex II. Sharma P, Maklashina E, Cecchini G, Iverson TM.Nat Commun. 2018 Jan 18;9(1):274. doi: 10.1038/s41467-017-02713-8.PMID: 29348404

Structural and biochemical analyses reveal insights into covalent flavinylation of the Escherichia coli Complex II homolog quinol:fumarate reductase. Starbird CA, Maklashina E, Sharma P, Qualls-Histed S, Cecchini G, Iverson TM.J Biol Chem. 2017 Aug 4;292(31):12921-12933. doi: 10.1074/jbc.M117.795120. Epub 2017 Jun 14.

Prior Publications

Pankaj Sharma*, Rachana Tomar*, Samir Kumar Nath, Shivpratap Singh Yadav, Maulik D. Badmalia, Ashish, Bishwajit Kundu (2020) Decoding hyperthermophilic capability of Pyrococcus Furiosus L-Asparaginase: short b-strands stitch reversible association Scientific Reports, (In press).

Navdeep Kaur*, Amin Sagar*, Pankaj Sharma*, Ashish Ganguly and Pratap Kumar Pati (2020) Structural insights into rice SalTol QTL located SALT protein, Scientific Reports, 10, 16589.

Maulik D. Badmalia, Pankaj Sharma, Shiv Pratap Singh Yadav, Shikha Singh, Neeraj Khatri, Renu Garg & Ashish (2018) Bonsai Gelsolin Survives Heat Induced Denaturation by Forming β-Amyloids which Leach Out Functional Monomer. Scientific Reports, 8, 12602.

Sharma, P., Verma, N., Singh, P.K., Korpole, S., Ashish. Characterization of heat induced spherulites of lysozyme provides new insight on amyloid initiation. Sci Rep. 2016 Mar 1;6:22475.

Arif, E.*, Sharma, P.*, Solanki, A., Mallik, L., Rathore, Y.S., Twal, W.O., Nath, S.K., Gandhi, D., Holzman, L.B., Ostap, E.M., Ashish, Nihalani, D. Structural analysis of the Myo1c and Neph1 complex provides insight into the intracellular movement of Neph1. Mol Cell Biol. 2016 May 16;36(11):1639-54.

Tomar, R.*, Sharma, P.*, Srivastava, A., Bansal, S., Ashish, Kundu, B. Structural and functional insights into an archaeal L-asparaginase obtained through the linker-less assembly of constituent domains. Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3187-97.

Mallik, L.*, Arif, E.*, Sharma, P., Rathore, Y.S., Wong, H.N., Holzman, L.B., Ashish, Nihalani, D. Solution structure analysis of cytoplasmic domain of podocyte protein Neph1 using small/wide angle x-ray scattering (SWAXS). J Biol Chem. 2012 Mar 16;287(12):9441-53.

*Authors contributed equally.