Prashant K. Singh, M.S.
Sr. Research Associate, Department of Pharmacology
- : prashant.k.singh@vanderbilt.edu
- : (615) 322-8721 Lab
- :
460 Robinson Research Building
2200 Pierce Ave
Nashville, Tennessee - 37232
My past and present primary research projects include bioenergetics, cell signaling, and viral diseases such as Ebola, HIV and more. I use cryo-EM to visualize the structures of macromolecular complexes and viral proteins, which provides insights into their function and potential targets for drug development. I also use animations and modeling programs like Blender, Chimera, and PyMOL to publish and present my findings in a visually engaging way.
In summary, my expertise lies at the intersection of cryo-EM, structural biology, and computational modeling. I am making constant contributions to understanding complex biological systems and developing new therapies for viral infections and other diseases.
Background
B. Tech, Meerut Institute of Engineering & Technology
M.S., Middle Tennessee State University
Awards
2024, Vanderbilt University Staff Research Award
2025, Vanderbilt University School of Medicine Edward E Price Award
Publications from the lab
Improving CryoEM maps of symmetry-mismatched macromolecular assemblies: A case study on the flagellar motor. J Struct Biol. 2025 Jun;217(2):108184. doi: 10.1016/j.jsb.2025.108184. Epub 2025 Mar 5.PMID: 40054642 Free article.
CryoEM structures reveal how the bacterial flagellum rotates and switches direction. Nat Microbiol. 2024 May;9(5):1271-1281. doi: 10.1038/s41564-024-01674-1. Epub 2024 Apr 17.PMID: 38632342 Free PMC article.
An evolving view of complex II-noncanonical complexes, megacomplexes, respiration, signaling, and beyond. J Biol Chem. 2023 Jun;299(6):104761. doi: 10.1016/j.jbc.2023.104761. Epub 2023 Apr 27.PMID: 37119852 Free PMC article. Review.
CryoEM structure of a post-assembly MS-ring reveals plasticity in stoichiometry and conformation. PLoS One. 2023 May 19;18(5):e0285343. doi: 10.1371/journal.pone.0285343. eCollection 2023.PMID: 37205674 Free PMC article.
An alternative N-terminal fold of the intestine-specific annexin A13a induces dimerization and regulates membrane-binding. J Biol Chem. 2019 Mar 8;294(10):3454-3463. doi: 10.1074/jbc.RA118.004571. Epub 2019 Jan 4.PMID: 30610115 Free PMC article
New crystal forms of the integral membrane Escherichia coli quinol:fumarate reductase suggest that ligands control domain movement. J Struct Biol. 2018 Apr;202(1):100-104. doi: 10.1016/j.jsb.2017.11.004. Epub 2017 Nov 20.PMID: 29158068 Free PMC article.
Structural basis of arrestin-3 activation and signaling. Nat Commun. 2017 Nov 10;8(1):1427. doi: 10.1038/s41467-017-01218-8.PMID: 29127291 Free PMC article.
Plasticity of the quinone-binding site of the complex II homolog quinol:fumarate reductase. J Biol Chem. 2013 Aug 23;288(34):24293-301. doi: 10.1074/jbc.M113.487082. Epub 2013 Jul 8.PMID: 23836905