Hamm Lab

Research Interests:

Coming from a purely pharmacological background with an emphasis on G protein-coupled receptors (GPCR) my general research interest lies in understanding how this very effective signaling module has been adapted to controlling signaling events across all systems in the body, by dissecting out the details through which they have diversified and distinguished themselves in each tissue. My post-doctoral work is focused on characterizing the signaling of a unique group of G protein-coupled receptors (GPCRs), the protease activated receptors (PAR) in two primary tissues: platelets and Human Umbilical Vein Endothelial Cells (HUVECs). I am interested in defining conventional paradigms of signaling through these receptors, differences in signaling pathways initiated by different receptor subtypes, and also functional selectivity displayed by these receptor in response to endogenous ligands and also demonstrating how these signaling paradigms contribute to primary cell physiology.

​Publications:

Duvernay MT, Temple KJ, Maeng JG, et al. Contributions of Protease-Activated Receptors PAR1 and PAR4 to Thrombin-Induced GPIIbIIIa Activation in Human Platelets. Mol Pharmacol. 2017;91(1):39-47.

Temple KJ, Duvernay MT, Maeng JG, et al. Identification of the minimum PAR4 inhibitor pharmacophore and optimization of a series of 2-methoxy-6-arylimidazo[2,1-b][1,3,4]thiadiazoles. Bioorg Med Chem Lett. 2016;26(22):5481-5486.

Temple KJ, Duvernay MT, Young SE, et al. Development of a Series of (1-Benzyl-3-(6-methoxypyrimidin-3-yl)-5-(trifluoromethoxy)-1H-indol-2-yl)methanols as Selective Protease Activated Receptor 4 (PAR4) Antagonists with in Vivo Utility and Activity Against γ-Thrombin. Journal of medicinal chemistry. 2016; 59(16):7690-5.

Oliver KH, Duvernay MT, Hamm HE, Carneiro AM. Loss of Serotonin Transporter Function Alters ADP-mediated Glycoprotein αIIbβ3 Activation through Dysregulation of the 5-HT2A Receptor. The Journal of biological chemistry. 2016; 291(38):20210-9.

Duvernay MT, Matafonov A, Lindsley CW, Hamm HE. Platelet Lipidomic Profiling: Novel Insight into Cytosolic Phospholipase A2α Activity and Its Role in Human Platelet Activation. Biochemistry. 2015; 54(36):5578-88.

A Novel and Selective PAR4 Antagonist: ML354.  Young SE, Duvernay MT, Schulte ML, Nance KD, Melancon BJ, Engers J, Wood MR, Hamm HE, Lindsley CW.  Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010-2013 Apr 15 [updated 2015 Feb 11].

Substituted indoles as selective protease activated receptor 4 (PAR-4) antagonists: Discovery and SAR of ML354. Wen W, Young SE, Duvernay MT, Schulte ML, Nance KD, Melancon BJ, Engers J, Locuson CW 2nd, Wood MR, Daniels JS, Wu W, Lindsley CW, Hamm HE, Stauffer SR.  Bioorg Med Chem Lett. 2014 Oct 1;24(19):4708-13

Racial differences in resistance to P2Y12 receptor antagonists in type 2 diabetic subjects.  Cleator JH, Duvernay MT, Holinstat M, Colowick NE, Hudson WJ, Song Y, Harrell FE, Hamm HE.  J Pharmacol Exp Ther. 2014 Oct;351(1):33-43.

Synthesis of indole derived protease-activated receptor 4 antagonists and characterization in human platelets. Young SE, Duvernay MT, Schulte ML, Lindsley CW, Hamm HE.  PLoS One. 2013 Jun 11;8(6):e65528.

The angiotensin II type 1 receptor C-terminal Lys residues interact with tubulin and modulate receptor export trafficking.  Zhang X, Wang H, Duvernay MT, Zhu S, Wu G.  PLoS One. 2013;8(2):e57805.

Alpha2B-adrenergic receptor interaction with tubulin controls its transport from the endoplasmic reticulum to the cell surface.  Duvernay MT, Wang H, Dong C, Guidry JJ, Sackett DL, Wu G.  J Biol Chem. 2011 Apr 22;286(16):14080-9.

ADP-ribosylation factors modulate the cell surface transport of G protein-coupled receptors.  Dong C, Zhang X, Zhou F, Dou H, Duvernay MT, Zhang P, Wu G.  J Pharmacol Exp Ther. 2010 Apr;333(1): 174-83.

Duvernay MT, Dong C, Zhang X, Robitaille M, Hébert TE, Wu G. A single conserved leucine residue in the first intracellular loop regulates ER export of G protein-coupled recetors. Traffic.2009,10:552-66

Duvernay MT, Dong C, Zhang X, Zhou F, Nichols CD, Wu G. Anterograde trafficking of G protein-coupled receptors: function of the C-terminal F(X)6LL motif in export from the endoplasmic reticulum. Mol Pharmacol. 2009, 75:751-61.

Dong C, Filipeanu CM, Duvernay MT, Wu G. Regulation of G protein-coupled receptor export trafficking. (Invited review). Biochim Biophys Acta. 2007, 1768:853-70.

Zhou F, Filipeanu CM, Duvernay MT, Wu G. Cell-surface targeting of alpha2-adrenergic receptors — inhibition by a transport deficient mutant through dimerization. Cell Signal. 2006, 18:18-27.

Duvernay MT, Filipeanu CM, Wu G. The regulatory mechanisms of export trafficking of G protein-coupled receptors. (Invited Review). Cell Signal. 2005, 17:1457-65.

Duvernay MT, Zhou F, Wu G. A conserved motif for the transport of G protein-coupled receptors from the Endoplasmic Reticulum to the Cell Surface. J Biol Chem. 2004, 279:30741-50.