Hamm Lab

Education:
MS, Physics/Mathematics (1959)
MS, Chemistry (1960)

Research Interests: 
Based on a multidisciplinary approach (synthetic methods in organic chemistry; biophysical and computational methods in structural biology: NMR, crystallization, optical spectrocopies; neutron scattering; homology modeling) our research is focused on two major pharmacological challenges: (i) regulation of chromatin acetylation through a synthetic strategy with novel inhibitors that synergize with major antitumoral drugs in cancer biology and therapy; (ii) search for antidepressant drugs of natural origin (terpenes) selectively acting on the kappa-opioid receptor.

Publications:

Gupta A, Gomes I, Bobeck EN, Fakira AK, Massaro NP, Sharma I, Cavé A, Hamm HE, Parello J, Devi LA. Collybolide is a novel biased agonist of κ-opioid receptors with potent antipruritic activity. Proc Natl Acad Sci U S A. 2016 May 24;113(21):6041-6. PubMed Central PMCID: PMC4889365.

Phyllochrysine et sécurine, alcaloïdes du Phyllantus discoides Muell. Arg. (Euphorbiacées), J. Parello , A. Melera et R. Goutarel. Bull. Soc. Chim., 1963 , 898 -910.

Couplage entre spins nucléaires en résonance magnétique nucléaire (RMN) et méthode de double résonance . J. Parello . Bull. Soc. Chim., 1964, 2033 -2062.

The structure of Sugar Osazones . L. Mester , E .Moczar and J . Parello . J. Amer. Chem. Soc., 1965 , 87, 596-599.

Etude des spectres de masse de la sécurinine et dérivés d’hydrogénation. H.E.Audier et J.Parello. Bull. Soc. Chim., 1968,1552-1559.

Alcaloïdes stéroïdiques -LXXV. Etude dichroïque du chromophore nitrone . J.Parello et X.Lusinchi. Tetrahedron. 1968, 24, 6747-6754.

Alcaloïdes steroïdiques LXXVIII . Etude dichroïque du chromophore amine tertiaire aliphatique . J. Parello et F. Picot . Tetrahedron Letters, 1968, 9, 5083-5086.

Alcaloides steroïdiques LXXIX – Photochimie de nitrones et d’oxazirannes steroïdiques . J. Parello , R . Beugelmans, P. Milliet et X. Lusinchi . Tetrahedron Letters, 1968, 9, 5081-5092.

N.M.R. studies on nitrogen-containing sugar derivatives substituted by15N. Part I. The nature of the chelate ring in sugar osazones . L.Mester, G.Vass, A.Stephen and J.Parello. Tetrahedron Letters,1968, 4053-4056.

Conformational studies on muscular parvalbumins. I. Optical rotatory dispersion and circular dichroism analysis. Parello J; Pechere J F Biochimie 1971, 53, 1079-83.

Oxazirannes. Obtention de lactames N-substitués par photolyse d’oxazirannes. E. Desherces, M. Rivière, J. Parello et A. Lattes, C.R.Acad. Sci. Paris, 1972, 275(C), 581-584.

Model studies in relation to the molecular structure of chromatin. Gourévitch M, Puigdomènech P, Cavé A; Etienne G; Méry J, Parello J Biochimie 1974, 56, 967-85.

Isolation and structural analysis of collybolide, a novel sesquiterpene extracted from Collybia maculata. Carbon-13 NMR of lactones. Bui, A. M.; Cave, A.; Janot, M. M.; Parello, J.; Potier, P.; Scheidegger, U. Tetrahedron 1974, 30, 1327-36.

Photolysis of oxaziridines. IV. Regioselectivity and solvent effects in the opening of oxaziridines at the spiranic junction. Oliveros-Desherces, E.; Riviere, M.; Parello, J.; Lattes, A. Tetrahedron Lett. 1975, 851-854.

Conformation mobility within the structure of muscular parvalbumins. An NMR study of the aromatic resonances of phenylalanine residues. Cavé A; Dobson CM; Parello J; Williams R J FEBS Lett. 1976, 65,190-194.

Superstructure of linear duplex DNA. Vollenweider, H. J.; Koller, T.; Parello, J.; Sogo, J. M. Proc. Nat. Acad. Sci. USA 1976, 73, 4125-4129.

A calcium-43 NMR study of the binding of calcium to parvalbumins. Parello, J.; Lilja, H.; Cave, A.; Lindman, B. FEBS Lett. 1978, 87, 191-195.

Chemical studies on histone acetylation using a synthetic peptide fragment of histone H4. Kervabon A; Parello J; Méry J FEBS Lett. 1979, 98, 152-156.

Na+ binding to parvalbumins studied by 23Na NMR. Parello J; Reimarsson P; Thulin E; Lindman B FEBS Lett. 1979, 100, 153-156.

Magnesium ion binding to parvalbumins studied by magnesium-25 and cadmium-113 NMR spectroscopy. Cave, A.; Parello, J.; Drakenberg, T.; Thulin, E.; Lindman, B. FEBS Lett. 1979, 100, 148-52.

Enzymatic deacetylation of a synthetic peptide fragment of histone H4. Kervabon A; Mery J; Parello J FEBS Lett. 1979, 106, 93-6.

Dynamic aspects of the structure of globular proteins by high resolution NMR spectroscopy. The fluid-like structure of the internal hydrophobic core of muscular parvalbumins. A. Cavé and J. Parello, in Les Houches, Session XXXIII, 1979. Membranes and Intercellular Communication, North-Holland Publishing Company, 1981, 197-227.

Effect of cations on the acetylation of chromatin in vitro. Dod B; Kervabon A; Parello J Eur. J. Biochem. 1982, 121, 401-405.

NMR studies of muscle proteins. Ribeiro A; Parello J; Jardetzky O. Prog. Biophys. Molec. Biol. 1984, 43, 95-160.

Metal-ion binding to parvalbumin. A 113Cd-n.m.r. study of the binding of different lanthanide ions. Drakenberg T; Sward M; Cave A; Parello J Biochem. J. 1985, 227, 711- 7.

A chromatin core particle obtained by selective cleavage of histones by clostripain. Dumuis-Kervabon A; Encontre I; Etienne G; Jauregui-Adell J; Mery J; Mesnier O; Parello J. EMBO J. 1986, 5, 1735-42.

Chromatin core particle obtained by selective cleavage of histones H3 and H4 by clostripain. Encontre I; Parello J. J. Mol. Biol. 1988, 202, 673-6.

Crystal structure determination and refinement of pike 4.10 parvalbumin (minor component from Esox lucius). Declercq J P; Tinant B; Parello J; Etienne G; Huber R., J. Mol. Biol., 1988, 202, 349-53.

Two-dimensional 1 H nuclear magnetic resonance study of pike pl 5.0 parvalbumin (Esox lucius). Sequential resonance assignments and folding of the polypeptide chain. Erratum in: J Mol Biol 1989 Aug 20;208(4):723-4 Padilla A; Cave A; Parello J., J. Mol. Biol., 1988, 204, 995-1017.

Ionic interactions with parvalbumins. Crystal structure determination of pike 4.10 parvalbumin in four different ionic environments. Declercq JP, Tinant B, Parello J, Rambaud J. J Mol Biol. 1991, 220,1017-1039.

Crystallization of catalytic subunit of adenosine cyclic monophosphate-dependent protein kinase. Zheng JH, Knighton DR, Parello J, Taylor SS, Sowadski JM. Methods Enzymol. 1991, 200, 508-521.

Mass spectrometry of proteins: studies of parvalbumins by plasma desorption, laser desorption and electrospray mass spectrometry. Roepstorff, P.; Klarskov, K.; Andersen, J.; Mann, M.; Vorm, O.; Etienne, G.; Parello, J. International Journal of Mass Spectrometry and Ion Processes 1991, 111, 151-172.

Homonuclear Three-Dimensional 1H NMR Spectroscopy of Pike Parvalbumin. Comparison of Short- and Medium-Range NOEs from 2D and 3D NMR. A. Padilla, G.W. Vuister, R. Boelens, G.J. Kleywegt, A. Cavé, J. Parello and R. Kaptein. J. Am. Chem. Soc. 1990, 112, 5024-5030.

Reisman, J., Jariel-Encontre, I., Hsu, V.L., Parello, J., Geiduschek, E.P., Kearns, D.R. (1991). Improving Two-dimensional 1H NMR NOESY Spectra of a Large Protein by Selective Deuteriation. J. Amer. Chem. Soc. 113, 2787-2789.

Crystallization studies of the catalytic subunit of cAMP-dependent protein kinase: crystals of murine recombinant catalytic subunit and a mutant, Cys 343—-Ser 1 diffract to 2.7 A resolution. Zheng J H; Knighton DR; Xuong N H; Parello J; Taylor S S; Sowadski J M., Acta Cryst. B, 1992, 48 ( Pt 2) 241-4.

Crystal structure of the unique parvalbumin component from muscle of the leopard shark (Triakis semifasciata). The first X-ray study of an alpha-parvalbumin. Roquet F; Declercq J P; Tinant B; Rambaud J; Parello J J Mol Biol 1992, 223, 705-20.

A 1 H-NMR study of the transcription factor 1 from Bacillus subtilis phage SPO1 by selective 2H-Iabeling. Complete assignment and structural analysis of the aromatic resonances for a 22-kDa homodimer. Reisman J M; Hsu V L; Jariel-Encontre I; Lecou C; Sayre M H; Kearns DR; Parello J., Eur. J. Biochem. 1993, 213, 865- 73.

Regioselective synthesis of inhibitors of histone acetyltransferase covalently linking spermidine to the S-terminus of coenzyme A. G. Roblot, R. Wylde, A. Martin and J. Parello,Tetrahedron 1993, 49, 6381-6398.

Proton and nitrogen NMR sequence-specific assignments and secondary structure determination of the Bacillus subtilis SPO1-encoded transcription factor 1. Jia X; Reisman J M; Hsu V L; Geiduschek E P; Parello J; Kearns D.R. Biochemistry 1994, 33, 8842-52.

Crystals of the cell-binding module of fibronectin obtained from a series of recombinant fragments differing in length. Dickinson CD; Gay DA; Parello J; Ruoslahti E; Ely K.R., J. Mol Biol 1994, 238, 123-7.

Evidence indicating proximity in the nucleosome between the histone H4 N termini and the globular domain of histone H1. Baneres J L; Essalouh L; Jariel-Encontre I; Mesnier O; Garrod S; Parello J., J. Mol. Biol. 1994, 243, 48-59.

Dynamics of a globular protein as studied by neutron scattering and solid-state NMR. Zanotti, J.-M.; Bellissent-Funel, M.-C.; Parello, J., Physica B: Condensed Matter 1997, 234-236,  228-230.

The N tails of histones H3 and H4 adopt a highly structured conformation in the nucleosome. Baneres J L; Martin A; Parello J. J. Mol. Biol., 1997, 273, 503-8.

The cation-binding domain from the alpha subunit of integrin alphaS beta1 is a minimal domain for fibronectin recognition. Baneres J L; Roquet F; Green M; LeCalvez H; Parello J., J. Biol. Chem. 1998, 273, 24744-53.

Ca2+/Mg2+ ex change in parvalbumin and other EF-hand proteins. A theoretical study. Allouche D; Parello J; Sanejouand Y H., J. Mol. Biol. 1999, 285, 857-73.

Two well-defined motifs in the cAMP-dependent protein kinase inhibitor (PKlalpha) correlate with inhibitory and nuclear export function. Hauer J A; Sarthe P; Taylor S S; Parello J; Padilla A. Prot. Science, 1999, 8, 545-53.

\Hydration-coupled dynamics in proteins studied by neutron scattering and NMR: the case of the typical EF-hand calcium-binding parvalbumin. Zanotti J M; Bellissent-Funel MC; Parello J., Biophys. J. 1999, 76, 2390-411.

Crystal structure of the EF-hand parvalbumin at atomic resolution (0.91 A) and at low temperature (100 K). Evidence for conformational multistates within the hydrophobic core. Declercq J P; Evrard C; Lamzin V; Parello J Prot. Science 1999, 8, 2194-204.

NMR and Hydration of Biomacromolecules in Aqueous Solutions. J. Parello, in Hydration Processes in Biology (M.-C. Bellissent-Funel, ed.), IOS Press, 1999, 379-385.

A minimized human integrin alpha(5)beta(1) that retains ligand recognition. Baneres J L; Roquet F; Martin A; Parello J., J. Biol. Chem. 2000, 275, 5888-903.

Durand D; Hullot P; Vidal J P; Girard J P; Baneres J L; Parello J; Muller A; Bonne C; Rossi J C., Leukotriene B4 photoaffinity probes: design, synthesis and evaluation of new arylazide-1,3-disubstituted cyclohexanes. Bioorg. Med. Chem. Lett. 2000, 10, 811-4.

Photochemical rearrangement of oxaziridines and nitrones in the hexahydroindole series: a convenient synthetic route to 1-azabicyclo[5.2.0]nonan-2-ones as novel RGD mimetics. Bourguet E; Baneres J L; Girard J P; Parello J; Vidal J P; Lusinchi X; Declercq J P., Org. Lett. 2001, 3, 3067-70.

A neutron scattering study of the histone sub-assemblies within the nucleosome core particle. J.-L. Banères, J. Parello, G. Zaccaï and D. Svergun, The ILL millenium symposium & european user meeting, Proceedings, ILL Neutrons for Science, 2001, 55-57.

Bourguet E, Baneres JL, Parello J, Lusinchi X, Girard JP, Vidal JP. Nonpeptide RGD antagonists: A novel class of mimetics, the 5,8-disubstituted 1-azabicyclo[5.2.0]nonan-2-one lactam. Bioorg Med Chem Lett. 2003;13,1561-4.

Banères, J.-L., Martin, A., Hullot, P., Girard, J.-P., Rossi, J.-C., & Parello, J. (2003). Structure-based analysis of GPCR function. Conformational adaptation of both agonist and receptor upon leukotriene B4 binding to recombinant BLT1. J. Mol. Biol., 329, 801-814.

Banères, J.-L., & Parello, J. (2003). Structure-based analysis of GPCR function. Evidence for a novel pentameric assembly between the dimeric leukotriene B4 receptor BLT1 and the G-protein. J. Mol. Biol., 329, 815-829.

Keya Bandyopadhyay, Casey Lee, Ali Haghighi, Jean-Louis Banères, Joseph Parello and Ruth A. Gjerset (2007). Serine Phosphorylation-Dependent Coregulation of Topoisomerase I by the p14ARF Tumor Suppressor. Biochemistry, 46 (49), 14325 -14334.

Preininger AM, Parello J, Meier SM, Liao G, Hamm HE (2008) Receptor-mediated changes at the myristoylated amino terminus of Galpha(i) proteins.Biochemistry 47,10281-93.

Martin, Damian, Laguerre, Parello, Pucci, Serre, Mary, Marie & Baneres (2009). Engineering a G protein-coupled receptor for structural studies: Stabilization of the BLT1 receptor ground state. Protein Science, 18, 727-734.