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Services & Instrumentation

The Proteomics Core offers a comprehensive set of services for analysis of proteins ranging from simple protein identifications and post-translational modification mapping to discovery-based global proteome comparisons and protein-protein interaction studies. State-of-the-art instrumentation, methods, and expertise are available to address almost any need. Our services include but are not limited to:

  • Basic protein/protein mixture identification (from gel bands or in solution)
  • Label-free differential expression discovery proteomics (spectral counting and peak areas-LFQ)
  • Label-based differential expression discovery proteomics (iTRAQ/TMT and SILAC)
  • Targeted protein quantitation (MRM, PRM, and AQUA)
  • Post-translational modification identification and characterization (individual proteins to proteome-wide)
  • Hydrogen-deuterium exchange (HDX) analysis for structural characterization of proteins and interactions

If your project does not fit readily into one of these categories or you are not certain of the best strategy, please do not hesitate to contact us and set up a consultation. Our associate directors, Drs. Rose and McDonald, have a combined 35 years of experience in the field and can help guide you through the entire process.

The Proteomics Core laboratory, located in 9110 MRB III of the Mass Spectrometry Research Center, has two Thermo Scientific LTQ ion trap mass spectrometers, five Thermo Scientific Q Exactive Orbitrap instruments (QE Classic, QE Plus (3), and QE HF), one Thermo Scientific LTQ Velos Orbitrap instrument equipped with electron transfer dissociation, and a Thermo Scientific Vantage triple quadrupole instrument. Each instrument is equipped with a nanoflow HPLC system for high sensitivity proteomics analysis. In addition, the Core has a Waters Xevo G2-XS QTOF mass spectrometer connected to a Waters HDX manager and LEAP Technologies liquid handling robot for automated HDX-MS analysis.