Skip to main content

Lang Lab

Stochastic but Highly Coordinated Protein Unfolding and Translocation by the ClpXP Proteolytic Machine

AUTHORS

Cordova J.C. , Olivares A.O. , Shin Y. , Stinson B.M. , Calmat S. , Schmitz K.R. , Aubin-Tam M-E. , Baker T.A. , Lang M.J. , Sauer R.T. . Cell. 2014 ; 158(3). 647-658

ABSTRACT

PDF  Link

ClpXP and other AAA+ proteases recognize, mechanically unfold, and translocate target proteins into a chamber for proteolysis. It is not known whether these remarkable molecular machines operate by a stochastic or sequential mechanism or how power strokes relate to the ATP-hydrolysis cycle. Single-molecule optical trapping allows ClpXP unfolding to be directly visualized and reveals translocation steps of ∼1-4 nm in length, but how these activities relate to solution degradation and the physical properties of substrate proteins remains unclear. By studying single-molecule degradation using different multidomain substrates and ClpXP variants, we answer many of these questions and provide evidence for stochastic unfolding and translocation. We also present a mechanochemical model that accounts for single-molecule, biochemical, and structural results for our observation of enzymatic memory in translocation stepping, for the kinetics of translocation steps of different sizes, and for probabilistic but highly coordinated subunit activity within the ClpX ring.