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Detecting Force-Induced Molecular Transitions with Florescence Resonant Energy Transfer


Tarsa P.B. , Brau R.R. , Barch M. , Ferrer J.M. , Freyzon Y. , Matsudaira P. , Lang M.J. . Angewandte Chemie, International Edition. 2007 ; 46(12). 1999-2001


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Don’t FRET: The first successful combination of optical-tweezers force microscopy and single-molecule fluorescence resonant energy transfer (FRET) is demonstrated with a force sensor based on a DNA hairpin (see picture). As the hairpin is opened and closed by the optical tweezers, the structural change is simultaneously monitored by the FRET emission from fluorescence labels.

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