Detecting Force-Induced Molecular Transitions with Florescence Resonant Energy Transfer
AUTHORS
Tarsa
P.B. ,
Brau
R.R. ,
Barch
M. ,
Ferrer
J.M. ,
Freyzon
Y. ,
Matsudaira
P. ,
Lang
M.J. .
Angewandte Chemie, International Edition. 2007 ; 46(12).
1999-2001
- PMID: 17279589[PubMed].
- PMCID: PMC3870335.
ABSTRACT
Don’t FRET: The first successful combination of optical-tweezers force microscopy and single-molecule fluorescence resonant energy transfer (FRET) is demonstrated with a force sensor based on a DNA hairpin (see picture). As the hairpin is opened and closed by the optical tweezers, the structural change is simultaneously monitored by the FRET emission from fluorescence labels.
