A major zebrafish polymorphism resource for genetic mapping
AUTHORS
Bradley
KMKevin M ,
Elmore
JBJ Bradford ,
Breyer
JPJoan P ,
Yaspan
BLBrian L ,
Jessen
JRJason R ,
Knapik
EWEla W ,
Smith
JRJeffrey R .
Genome biology. 2007 ; 8(4).
R55
- PMID: 17428331[PubMed].
- PMCID: PMC1896001.
ABSTRACT
We have identified 645,088 candidate polymorphisms in zebrafish and observe a single nucleotide polymorphism (SNP) validation rate of 71% to 86%, improving with polymorphism confidence score. Variant sites are non-random, with an excess of specific novel T- and A-rich motifs. We positioned half of the polymorphisms on zebrafish genetic and physical maps as a resource for positional cloning. We further demonstrate bulked segregant analysis using the anchored SNPs as a method for high-throughput genetic mapping in zebrafish.