Protocols
- Protocols
- Competent Cells Protocol
- hTERT-RPE serum starvation for synchronization
- Immunofluorescence Protocol
- hTERT-RPE serum starvation for synchronization
- Flow Cytometry Manuals
- Fork regression/restoration/footprinting
- SMARCAL1 DNA binding ATPase assays
- PI and BrdU Flow Cytometry Protocols
- Rong’s 293T suspension+mass spec
- Lysis with DNAse for all nuclear proteins including histones
- DNA Combing Protocol
- DNA fiber labeling
- Comet Assay
- Nascent ssDNA IF assay
- 53BP1 foci
- Retro/Lentivirus production
- PEI transfection of 293T cells
- siRNA transfection optimization
- Flag-His purification mammalian cells
- ATRflox cells Cre
- ATR kinase assay
- G2/M checkpoint assay
- Freezing/Thawing cells in Liquid Nitrogen
- RPA-ssDNA pull down
- GST protein purification from bacteria
- Bacterial expression/His purification
- HeLa siRNA/plasmid transfection
- Protein solubility test in E.coli
Cell Culture and related Protocols
FreeStyle-293-transfection-PEI
Tissue Culture plate sizes/cell numbers for transfections
siRNA Transfection cheat sheet
hTERT serum starvation for synchronization
siRNA transfection optimization
HeLa siRNA/plasmid transfection
Freezing/Thawing cells in Liquid Nitrogen
CRISPR-Cas9 Protocols Thomas (download word doc)
DNA damage, cell cycle, and related measurement protocols
Protein purification/extraction and related protocols
Protein solubility test in E.coli
GST protein purification from bacteria
Bacterial expression/His purification
Mammalian Cell Lysis with DNAse for all nuclear proteins including histones
Rong's 293T suspension+mass spec
Chromatin-Fractionation-Protocol-KJ (Kareem used for HMCES)
Chromatin-Fractionation-Protocol-Mendez-and-STillman
FLAG-purification-from-293T-cells-MA
Other assays and protocols
SMARCAL1 DNA binding ATPase assays
Fork regression/restoration/footprinting