Lysis with DNAse for all nuclear proteins including histones

From Kareem:

Lysis Buffer:
50mM Tris pH 8
150mM NaCl
0.5% NP40

Before use, supplement with 1mM MgCl2 and 1uL/mL Pierce Universal Nuclease for Cell Lysis. Incubate on ice for 30min. Spin.

This nuclease degrades both DNA and RNA. I use it in my IPs to get the chromatin bound HMCES. It is able to free all of the HMCES from the chromatin fraction.

Add protease and phosphatase inhibitors as appropraite.

Will release histones from DNA.