1. Lipid Mediator Biosynthesis: mechanisms of oxygenation of polyunsaturated fatty acids
2. Role of the 12R-Lipoxygenase Pathway in Skin Barrier Formation
LOX action in the mammalian epidermis: CerEOS to ox-CerEOS to bound CerOS. Oxidation of CerEOS containing esterified linoleic acid is initiated by 12R-LOX and its 9-hydroperoxy product is isomerized by Epidermal Lipoxygenase-3 (eLOX3) to form the epoxy-hydroxy product illustrated above. How this leads eventually to covalent binding of the ceramide is under investigation. Our 2019/2020 paper in the Journal of Clinical Investigation reports a new step in the pathway involving the short-chain dehydrogenase/reductase SDR9C7.