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Sando Lab

Essential Role of Latrophilin-1 Adhesion GPCR Nanoclusters in Inhibitory Synapses

AUTHORS

Matúš DDaniel , Lopez JMJaybree M , Sando RCRichard C , Südhof TCThomas C . The Journal of neuroscience : the official journal of the Society for Neuroscience. 2024 6 5; 44(23).

ABSTRACT

Latrophilin-1 (Lphn1, aka CIRL1 and CL1; gene symbol ) is an adhesion GPCR that has been implicated in excitatory synaptic transmission as a candidate receptor for α-latrotoxin. Here we analyzed conditional knock-in/knock-out mice for Lphn1 that contain an extracellular myc epitope tag. Mice of both sexes were used in all experiments. Surprisingly, we found that Lphn1 is localized in cultured neurons to synaptic nanoclusters that are present in both excitatory and inhibitory synapses. Conditional deletion of Lphn1 in cultured neurons failed to elicit a detectable impairment in excitatory synapses but produced a decrease in inhibitory synapse numbers and synaptic transmission that was most pronounced for synapses close to the neuronal soma. No changes in axonal or dendritic outgrowth or branching were observed. Our data indicate that Lphn1 is among the few postsynaptic adhesion molecules that are present in both excitatory and inhibitory synapses and that Lphn1 by itself is not essential for excitatory synaptic transmission but is required for some inhibitory synaptic connections.

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