The bacterial replisome has factory-like localization

AUTHORS

Mangiameli SMSarah M , Cass JAJulie A , Merrikh HHoura , Wiggins PAPaul A . Current genetics. 2018 4 9; 64(5). 1029-1036

PMID: 29632994[PubMed].

ABSTRACT

DNA replication is essential to cellular proliferation. The cellular-scale organization of the replication machinery (replisome) and the replicating chromosome has remained controversial. Two competing models describe the replication process: In the track model, the replisomes translocate along the DNA like a train on a track. Alternately, in the factory model, the replisomes form a stationary complex through which the DNA is pulled. We summarize the evidence for each model and discuss a number of confounding aspects that complicate interpretation of the observations. We advocate a factory-like model for bacterial replication where the replisomes form a relatively stationary and weakly associated complex that can transiently separate.

Tags: 2018