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Single-molecule investigations of single-chain cellulose biosynthesis


Hilton MAMark A , Manning HWHarris W , Górniak IIreneusz , Brady SKSonia K , Johnson MMMadeline M , Zimmer JJochen , Lang MJMatthew J . Proceedings of the National Academy of Sciences of the United States of America. 2022 09 26; 119(40). e2122770119


Cellulose biosynthesis in sessile bacterial colonies originates in the membrane-integrated bacterial cellulose synthase (Bcs) AB complex. We utilize optical tweezers to measure single-strand cellulose biosynthesis by BcsAB from . Synthesis depends on uridine diphosphate glucose, Mg, and cyclic diguanosine monophosphate, with the last displaying a retention time of ∼80 min. Below a stall force of 12.7 pN, biosynthesis is relatively insensitive to force and proceeds at a rate of one glucose addition every 2.5 s at room temperature, increasing to two additions per second at 37°. At low forces, conformational hopping is observed. Single-strand cellulose stretching unveiled a persistence length of 6.2 nm, an axial stiffness of 40.7 pN, and an ability for complexes to maintain a tight grip, with forces nearing 100 pN. Stretching experiments exhibited hysteresis, suggesting that cellulose microstructure underpinning robust biofilms begins to form during synthesis. Cellohexaose spontaneously binds to nascent single cellulose strands, impacting polymer mechanical properties and increasing BcsAB activity.