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Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis


AUTHORS

Snider CEChloe E , Willet AHAlaina H , Chen JSJun-Song , Arpağ GGöker , Zanic MMarija , Gould KLKathleen L . The Journal of cell biology. 2017 08 07; 216(10). 3041-3050

ABSTRACT

Many eukaryotic cells divide by assembling and constricting an actin- and myosin-based contractile ring (CR) that is physically linked to the plasma membrane (PM). In this study, we report that cells lacking , which encodes a conserved PM scaffold for the phosphatidylinositol-4 kinase Stt4, build CRs that can slide away from the cell middle during anaphase in a myosin V-dependent manner. The Efr3-dependent CR-anchoring mechanism is distinct from previously reported pathways dependent on the Fes/CIP4 homology Bin-Amphiphysin-Rvs167 (F-BAR) protein Cdc15 and paxillin Pxl1. In , the concentrations of several membrane-binding proteins were reduced in the CR and/or on the PM. Our results suggest that proper PM lipid composition is important to stabilize the central position of the CR and resist myosin V-based forces to promote the fidelity of cell division.