Deep sequencing of target linkage assay-identified regions in familial breast cancer: methods, analysis pipeline and troubleshooting.
AUTHORS
Rosa-Rosa
JMJuan Manuel ,
Gracia-Aznárez
FJ Francisco Javier ,
Hodges
E Emily ,
Pita
G Guillermo ,
Rooks
M Michelle ,
Xuan
Z Zhenyu ,
Bhattacharjee
A Arindam ,
Brizuela
L Leonardo ,
Silva
JM José M ,
Hannon
GJ Gregory J ,
Benitez
J Javier .
PloS one. 2010 ; 5(4).
e9976
- PMID: 20368986[PubMed].
- PMCID: PMC2848842.
ABSTRACT
The classical candidate-gene approach has failed to identify novel breast cancer susceptibility genes. Nowadays, massive parallel sequencing technology allows the development of studies unaffordable a few years ago. However, analysis protocols are not yet sufficiently developed to extract all information from the huge amount of data obtained.