Gurevich Lab

Research Description

Sue joined the lab in March of 2002 as a graduate student and defended her thesis in August of 2005. She stayed for another year as a post-doc to finish numerous projects. Sue worked on various aspects of arrestin structure and function, using a variety of methods from hard-core biochemistry and biophysics to visual arrestin translocation studies in mice in vivo. Sue published 22 papers and book chapters from my lab, with a few more in the pipeline.

Sue is currently Assistant Professor of Biology, Carroll University, Waukesha, WI (2009-present)

Publications from the lab:

Chen, Q., Zhuo, Y., Sharma, P., Perez, I., Francis, D.J., Chakravarthy, S., Vishnivetskiy, S.A., Berndt, S., Hanson, S.M., Zhan, X., Brooks, E.K., Altenbach, C., Hubbell, W.L., Klug, C.S., Iverson, T.M., Gurevich, V.V. An eight amino acid segment controls oligomerization and preferred conformation of the two non-visual arrestins. J Mol Biol 433 (4), 166790; doi: 10.1016/j.jmb.2020.166790 (2021).

Chen, Q., Zhuo, Y., Kim, M., Hanson, S.M., Vishnivetskiy, S.A., Altenbach, C., Klug, C.S., Hubbell, W.L., and Gurevich, V.V. Self-association of arrestin family members. In: Arrestins – Pharmacology and Therapeutic Potential. Handb Exp Pharmacol 219, p. 205-223, Springer-Verlag, Berlin-Heidelberg (2014).

Song, X., Seo, J., Baameur, F., Vishnivetskiy, S.A., Chen, Q., Kook, S., Kim, M., Brooks, E.K., Altenbach, C., Hong, Y., Hanson, S.M., Palazzo, M.C., Chen, J., Hubbell, W.L., Gurevich, E.V., Gurevich, V.V. Rapid degeneration of rod photoreceptors expressing self-association-deficient arrestin-1 mutant. Cell Signal 25, 2613-2624 (2013).

Kim, M., Vishnivetskiy, S.A., Van Eps, N., Alexander, N.S., Cleghorn, W.M., Zhan, X., Hanson, S.M., Morizumi, T., Ernst, O.P., Meiler*, J., Gurevich*, V.V., Hubbell*, W.L. (*corresponding authors) Conformation of receptor-bound visual arrestin. Proc. Natl. Acad. Sci. USA 109, 18407-18412 (2012).

Gurevich, V.V., Hanson, S.M., Song, X., Vishnivetskiy, S.A., Gurevich, E.V. The functional cycle of visual arrestins in photoreceptor cells. Prog Retin Eye Res 30, 405-430 (2011).

Coffa, S., Breitman, M., Hanson, S.M., Callaway, K., Kook, S., Dalby, K.N., Gurevich, V.V. The effect of arrestin conformation on the recruitment of C-Raf1, MEK1, and ERK1/2 activation. PLoS One 6(12), e28723 (2011).

Vishnivetskiy, S.A., Gimenez, L.E., Francis, D.J., Hanson, S.M., Hubbell, W.L., Klug, C.S., Gurevich, V.V. Few residues within an extensive binding interface drive receptor interaction and determine the specificity of arrestin proteins. J Biol Chem 286, 24288-24299 (2011).

Kim, M., Hanson, S.M., Vishnivetskiy, S.A., Song, X., Cleghorn, W.M., Hubbell, W.L., Gurevich, V.V. Robust self-association is a common feature of mammalian visual arrestin-1. Biochemistry 50, 2235-2242 (2011).

Vishnivetskiy, S.A., Francis, D., Van Eps, N., Kim, M., Hanson, S.M., Klug, C.S., Hubbell, W.L, Gurevich, V.V. The role of arrestin a-helix I in receptor binding. J Mol Biol 395, 42-54 (2010).

Hanson, S.M., Dawson, E.S., Francis, D.J., Van Eps, N., Klug, C.S., Hubbell, W.L., Meiler, J., and Gurevich, V.V. A Model for the Solution Structure of the Rod Arrestin Tetramer. Structure 16, 924-934 (2008).

Hanson, S.M., Vishnivetskiy, S.A., Hubbell, W.L., and Gurevich, V.V. The opposing effects of inositol hexakisphosphate (IP6) on rod arrestin and arrestin2 self-association. Biochemistry 47, 1070-5 (2008).

Gurevich, V.V., Hanson, S.M. , Gurevich, E.V., Vishnivetskiy, S.A . How Rod Arrestin Achieved Perfection: Regulation of its Availability and Binding Selectivity. In: Signal Transduction in the retina (Kisselev, O., and Fliesler, S.J., Eds), pp 55-88. Methods in Signal Transduction Series, CRC Press, Boca Raton, FL (2007).

Hanson, S.M. , Gurevich, E.V., Vishnivetskiy, S.A., Ahmed, M.R., Song, X., and Gurevich, V.V. Each rhodopsin molecule binds its own arrestin. Proc. Natl. Acad. Sci. USA 104 , 3125-3128 (2007).

Chan, S., Rubin, W.W., Mendez, A., Liu, X., Song, X., Hanson, S.M. , Craft, C.M., Gurevich, V.V., Burns, M.E., and Chen, J. Functional comparisons of visual arrestins in rod photoreceptors of transgenic mice. Invest Ophthalmol Vis Sci 48 , 1968-1075 (2007).

Hanson, S.M. , Van Eps, N., Francis, D.J., Altenbach, C., Vishnivetskiy, S.A., Arshavsky, V.Y., Klug, C.S., Hubbell, W.L., and Gurevich, V.V. Structure and Function of the Visual Arrestin Oligomer. EMBO J 26 , 1726-36 (2007).

Hanson, S.M., Cleghorn, W.M., Francis, D.J., Vishnivetskiy, S.A., Raman, D., Song, X., Nair, K.S., Slepak, V.Z., Klug, C.S., and Gurevich, V.V. Arrestin mobilizes signaling proteins to the cytoskeleton and redirects their activity. J Mol Biol 368 , 375-387 (2007).

Hanson, S.M. , and Gurevich, V.V. The differential engagement of arrestin surface charges by the various functional forms of the receptor. J Biol Chem 281, 3458-3462 (2006).

Hanson, S.M. , Francis, D.J., Vishnivetskiy, S.A., Klug, C.S., and Gurevich, V.V. Visual arrestin binding to microtubules involves a distinct conformational change. J Biol Chem 281 , 9765-9772 (2006).

Hanson, S.M. , Francis, D.J., Vishnivetskiy, S.A., Kolobova, E.A., Hubbell, W.L., Klug, C.S., and Gurevich, V.V. Differential interaction of spin labeled arrestin with inactive and active phosphorhodopsin. Proc Natl Acad Sci USA 103 , 4900-4905 (2006).

Wu, N., Macion-Dazard, R., Nithianantham, S., Xu, Z., Hanson, S.M., Vishnivetskiy, S.A., Gurevich, V.V., Thibonnier, M., and Shoham, M. Soluble mimics of the cytoplasmic face of the human V1-vascular vasopressin receptor bind arrestin2 and calmodulin. Mol Pharmacol 70 , 249-258 (2006).

Wu, N., Hanson, S.M. , Francis, D.J., Vishnivetskiy, S.A., Thibonnier, M., Klug, C.S., Shoham, M., and Gurevich, V. V. Arrestin binding to calmodulin: a direct interaction between two ubiquitous signaling proteins. J Mol Biol 364 , 955-963 (2006).

Sutton, R.B., Vishnivetskiy, S.A., Robert, J., Hanson, S.M. , Raman, D., Knox, B.E., Kono, M., Navarro, J., and Gurevich, V.V. Crystal structure of cone arrestin at 2.3A: Evolution of receptor specificity. J Mol Biol 354 , 1069-1080 (2005).

Nair, K.S.*, Hanson, S.M.* , Mendez, A., Gurevich, E.V., Kennedy, M.J., Shestopalov, V.I., Vishnivetskiy, S.A., Chen, J., Hurley, J.B., Gurevich, V.V., and Slepak, V.Z. Light-dependent redistribution of arrestin in vertebrate rods is an energy-independent process governed by protein-protein interactions. Neuron 46 , 555-567 (2005). *co-first author

Nair, K.S., Hanson, S.M. , Kennedy, M.J., Hurley, J.B., Gurevich, V.V., and Slepak, V.Z. Direct binding of visual arrestin to microtubules determines the differential subcellular localization of its splice variants in rod photoreceptors. J Biol Chem 279 , 41240-41248 (2004).