Zac’s paper online at MBoC
Zac Elmore's thesis work is now available online at MBoC. Zac determined how S. pombe casein kinases Hhp1/2 are targeted to the spindle pole body: localization information is in the kinase domain. Zac collaborated with co-grad student Rodrigo Guillen to show this is a conserved feature of casein kinases, since human CK1 delta/epsilon also use kinase domain to localize to centrosomes.
Click here to read the paper
Figure reproduced from: Elmore et al., MBoC, 2018
Residues at the base of the Hhp1 catalytic domain are critical for SPB localization. (A) Homology model of Hhp1 catalytic domain generated from Phyre2 software and visualized with MacPymol. Residues critical for Hhp1 SPB localization are in blue. (B) Live-cell imaging of endogenously tagged Hhp1- (R261E)-mNG and Hhp1-(R272E K273E)-mNG with Sid4-RFP. Scale bars, 5 µm.