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Cortez Lab

Cortez Lab Publications

2023

Adolph Madison , Garje Atharv , Balakrishnan Swati , Morati F , Modesti M , Chazin WJ , Cortez David , CRISPR-dependent Base Editing Screens Identify Separation of Function Mutants of RADX with Altered RAD51 Regulatory Activity Journal of Molecular Biology. 2023 ;
Rua-Fernandez Jorge , Lovejoy Courtney , Mehta Kavi , Paulin Katherine , Toudji YT , Giansanti Celeste , Eichman Brandt , Cortez David , Self-reversal facilitates the resolution of HMCES DNA-protein crosslinks in cells Cell Reports. 2023 ;
Bhowmick Rahul , Mehta P.M. , Ledrup Mars , Cortez David , Integrator facilitates RNAPII removal to prevent transcription-replication collisions and genome instability Molecular Cell. 2023 Jun 6;
Kavlashvili Tata , Liu Wenpeng , Mohamed Taha , Cortez David , Dewar James , Replication fork uncoupling causes nascent strand degradation and fork reversal Nature Structural and Molecular Biology. 2023 Jan 30;
Liu Wenpeng , Saito Yuichiro , Jackson Jessica , Bhowmick Rahul , Kanemaki Masato , Vindigni Alessandro , Cortez David , RAD51 bypasses the CMG helicase to promote replication fork reversal Science. 2023 Apr 28; 380 382-387

2022

Paulin Katherine , Cortez David , Eichman Brandt , The SOS response-associated peptidase (SRAP) domain of YedK catalyzes ring opening of abasic sites and reversal of its DNA-protein cross-link Journal of Biological Chemistry. 2022 Sep ;
Ravenstein Sabrina , Mehta Kavi , Kavlashvili Tamar , Byl Jo Anne , Zhao Runxiang , Osheroff Neil , Cortez David , Dewar M , Topoisomerase II poisons inhibit vertebrate DNA replication through distinct mechanisms EMBO Journal. 2022 May 16; 41
Mehta Kavi , Thada Vaughn , Zhao Runxiang , Krishnamoorthy Archana , Leser Michael , Rose Lindsey , cortez David , CHK1 phosphorylates PRIMPOL to promote replication stress tolerance Science Advances. 2022 Mar 30;
Mohamed Taha , Adolph B , Cortez David , Journal of Biological Chemistry. 2022 ;

2021

Krishnamoorthy Archana , Jackson Jessica , Mohamed Taha , Adolph Madison , Vindigni Alesandro , Cortez David , RADX prevents genome instability by confining replication fork reversal to stalled forks Molecular Cell. 2021 ;
Adolph B , Mohamed M , Balakrishnan Swati , Due C , Morati F , Modesti M , Greene C , Chazin J , Cortez David , RADX controls RAD51 filament dynamics to regulate replication fork stability Molecular Cell. 2021 ;

2020

Liu Wenpeng , Krishnamoorthy Archana , Zhao Runxiang , Cortez David , Two replication fork remodeling pathways generate nuclease substrates for distinct fork protection factors. Science Advances. 2020 ; 6 eabc3598
Mehta PM , Lovejoy A , Zhao Runxiang , Heintzman Darren , Cortez David , HMCES Maintains Replication Fork Progression and Prevents Double-Strand Breaks in Response to APOBEC Deamination and Abasic Site Formation Cell Reports. 2020 ;
Thompson S , Cortez David , New Insights Into Abasic Site Repair and Tolerance DNA Repair. 2020 ;

2019

Wessel R , Mohni N , Luzwick W , Dungrawala Huzefa , Cortez David , Functional Analysis of the Replication Fork Proteome Identifies BET Proteins as PCNA Regulators Cell Reports. 2019 Sep 24; 28 3497-3509
Thompson S , Amidon M , Mohni N , Cortez David , Eichman F , Protection of abasic sites during DNA replication by a stable thiazolidine protein-DNA cross-link Nature Structure & Molecular Biology. 2019 ;
Cortez David , Replication-Coupled DNA Repair Molecular Cell. 2019 ; 74 866-876
Thada Vaughn , Cortez David , Common motifs in ETAA1 and TOPBP1 required for ATR kinase activation Journal of Biological Chemistry. 2019 Apr 2;
Bass Thomas , Cortez David , Quantitative phosphoproteomics reveals mitotic function of the ATR activator ETAA1 Journal of Cell Biology. 2019 ;
Mohni Kareem , Wessel Sarah , Zhao Runxiang , Wojciechowski Andrea , Luzwick Jessica , Layen Hillary , Eichman Brandt , Thompson Petria , Mehta Kavi , Cortez David , HMCES Maintains Genome Integrity by Shielding Abasic Sites in Single-Strand DNA Cell. 2019 ;

2018

Saldivar J , Hamperl S , Bocek MJ , Chung M , Bass TE , Cisneros-Soberanis F , Samejima K , Xie L , Paulsen JR , Earnshaw WC , Cortez D , Meyer T , Cimprich KA , An intrinsic S/G2 checkpoint enforced by ATR Science. 2018 Aug ; 361 806-810
Bhat KP , Krishnamoorthy A , Dungrawala H , Garcin EB , Modesti M , Cortez D , RADX Modulates RAD51 Activity to Control Replication Fork Protection Cell Reports. 2018 Jul ; 24 (3). 538-547
Carvajal-Maldonado D , Byrum AK , Jackson J , Wessel S , Lemon D , Guitton-Sert L , Quinet A , Tirman S , Graziano S , Masson JY , Cortez D , Gonzalo S , Mosammapararast N , Vindigni A , Perturbing cohesin dynamics drives MRE11 nuclease-dependent replication fork slowing Nucleic Acids Research. 2018 Jun ;
Bhat Kamakoti , Cortez David , RPA and RAD51: fork reversal, fork protection, and genome stability. Nature Structural and Molecular Biology. 2018 ;

2017

Puccetti MV , Fischer MA , Arrate MP , Boyd KL , Duszynski RJ , Bétous R , Cortez D , Eischen CM , Defective replication stress response inhibits lymphomagenesis and impairs lymphocyte reconstitution. Oncogene. 2017 May 4; 36 2553-2564
Reynolds JJ , Bicknell LS , Carroll P , Higgs MR , Shaheen R , Murray JE , Papadopoulos DK , Leitch A , Murina O , Tarnauskaitė Ž , Wessel SR , Zlatanou A , Vernet A , von Kriegsheim A , Mottram RM , Logan CV , Bye H , Li Y , Brean A , Maddirevula S , Challis RC , Skouloudaki K , Almoisheer A , Alsaif HS , Amar A , Prescott NJ , Bober MB , Duker A , Faqeih E , Seidahmed MZ , Al Tala S , Alswaid A , Ahmed S , Al-Aama JY , Altmüller J , Al Balwi M , Brady AF , Chessa L , Cox H , Fischetto R , Heller R , Henderson BD , Hobson E , Nürnberg P , Percin EF , Peron A , Spaccini L , Quigley AJ , Thakur S , Wise CA , Yoon G , Alnemer M , Tomancak P , Yigit G , Taylor AM , Reijns MA , Simpson MA , Cortez D , Alkuraya FS , Mathew CG , Jackson AP , Stewart GS , Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism. Nature genetics. 2017 Feb 13;
Dungrawala H , Bhat KP , Le Meur R , Chazin WJ , Ding X , Sharan SK , Wessel SR , Sathe AA , Zhao R , Cortez D , RADX Promotes Genome Stability and Modulates Chemosensitivity by Regulating RAD51 at Replication Forks. Molecular cell. 2017 Jul 19;
Cortez D , Proteomic Analyses of the Eukaryotic Replication Machinery. Methods in enzymology. 2017 ; 591 33-53
Poole LA , Cortez D , Functions of SMARCAL1, ZRANB3, and HLTF in maintaining genome stability. Critical reviews in biochemistry and molecular biology. 2017 Dec ; 52 (6). 696-714
Vujanovic M , Krietsch J , Raso MC , Terraneo N , Zellweger R , Schmid JA , Taglialatela A , Huang JW , Holland CL , Zwicky K , Herrador R , Jacobs H , Cortez D , Ciccia A , Penengo L , Lopes M , Replication Fork Slowing and Reversal upon DNA Damage Require PCNA Polyubiquitination and ZRANB3 DNA Translocase Activity. Molecular cell. 2017 Sep 7; 67 (5). 882-890.e5
Saldivar JC , Cortez D , Cimprich KA , The essential kinase ATR: ensuring faithful duplication of a challenging genome. Nature reviews. Molecular cell biology. 2017 Oct ; 18 (10). 622-636

2016

Bass TE , Luzwick JW , Kavanaugh G , Carroll C , Dungrawala H , Glick GG , Feldkamp MD , Putney R , Chazin WJ , Cortez D , ETAA1 acts at stalled replication forks to maintain genome integrity. Nature cell biology. 2016 Oct 10;
Ray Chaudhuri A , Callen E , Ding X , Gogola E , Duarte AA , Lee JE , Wong N , Lafarga V , Calvo JA , Panzarino NJ , John S , Day A , Crespo AV , Shen B , Starnes LM , de Ruiter JR , Daniel JA , Konstantinopoulos PA , Cortez D , Cantor SB , Fernandez-Capetillo O , Ge K , Jonkers J , Rottenberg S , Sharan SK , Nussenzweig A , Replication fork stability confers chemoresistance in BRCA-deficient cells. Nature. 2016 Jul 21; 535 (7612). 382-7
Poole LA , Cortez D , SMARCAL1 and telomeres: Replicating the troublesome ends. Nucleus (Austin, Tex.). 2016 May 3; 7 (3). 270-4
Badu-Nkansah A , Mason AC , Eichman BF , Cortez D , Identification of a Substrate Recognition Domain in the Replication Stress Response Protein Zinc Finger Ran-binding Domain-containing Protein 3 (ZRANB3). The Journal of biological chemistry. 2016 Apr 8; 291 (15). 8251-7

2015

Kavanaugh G , Zhao R , Guo Y , Mohni KN , Glick G , Lacy ME , Hutson MS , Ascano M , Cortez D , Enhancer of Rudimentary Homolog affects the replication stress response through regulation of RNA processing. Molecular and cellular biology. 2015 Jun 22;
Mohni KN , Thompson PS , Luzwick JW , Glick GG , Pendleton CS , Lehmann BD , Pietenpol JA , Cortez D , A Synthetic Lethal Screen Identifies DNA Repair Pathways that Sensitize Cancer Cells to Combined ATR Inhibition and Cisplatin Treatments. PloS one. 2015 ; 10 (5). e0125482
Cortez D , Preventing replication fork collapse to maintain genome integrity. DNA repair. 2015 May 1;
Carroll C , Hunley TE , Guo Y , Cortez D , A novel splice site mutation in SMARCAL1 results in aberrant exon definition in a child with schimke immunoosseous dysplasia. American journal of medical genetics. Part A. 2015 May 5;
Ning B , Feldkamp MD , Cortez D , Chazin WJ , Friedman KL , Fanning E , Simian virus Large T antigen interacts with the N-terminal domain of the 70 kD subunit of Replication Protein A in the same mode as multiple DNA damage response factors. PloS one. 2015 ; 10 (2). e0116093
Bhat KP , Bétous R , Cortez D , High-affinity DNA-binding domains of replication protein A (RPA) direct SMARCAL1-dependent replication fork remodeling. The Journal of biological chemistry. 2015 Feb 13; 290 (7). 4110-7
Dungrawala H , Cortez D , Purification of proteins on newly synthesized DNA using iPOND. Methods in molecular biology (Clifton, N.J.). 2015 ; 1228 123-31
Dungrawala H , Rose KL , Bhat KP , Mohni KN , Glick GG , Couch FB , Cortez D , The Replication Checkpoint Prevents Two Types of Fork Collapse without Regulating Replisome Stability. Molecular cell. 2015 Sep 17; 59 (6). 998-1010
Poole LA , Zhao R , Glick GG , Lovejoy CA , Eischen CM , Cortez D , SMARCAL1 maintains telomere integrity during DNA replication. Proceedings of the National Academy of Sciences of the United States of America. 2015 Dec 1; 112 (48). 14864-9
Cortez D , Zhou Z , Sanchez Y , Stephen Elledge and the DNA damage response. DNA repair. 2015 Nov ; 35 156-7
Kavanaugh G , Ye F , Mohni KN , Luzwick JW , Glick G , Cortez D , A whole genome RNAi screen identifies replication stress response genes. DNA repair. 2015 Nov ; 35 55-62

2014

Sowd GA , Mody D , Eggold J , Cortez D , Friedman KL , Fanning E , SV40 utilizes ATM kinase activity to prevent non-homologous end joining of broken viral DNA replication products. PLoS pathogens. 2014 Dec ; 10 (12). e1004536
Luzwick JW , Nam EA , Zhao R , Cortez D , Mutation of Serine 1333 in the ATR HEAT Repeats Creates a Hyperactive Kinase. PloS one. 2014 ; 9 (6). e99397
Mason AC , Rambo RP , Greer B , Pritchett M , Tainer JA , Cortez D , Eichman BF , A structure-specific nucleic acid-binding domain conserved among DNA repair proteins. Proceedings of the National Academy of Sciences of the United States of America. 2014 May 27; 111 (21). 7618-23
Mohni KN , Kavanaugh GM , Cortez D , ATR Pathway Inhibition Is Synthetically Lethal in Cancer Cells with ERCC1 Deficiency. Cancer research. 2014 May 15; 74 (10). 2835-45
Couch FB , Cortez D , Fork reversal, too much of a good thing. Cell cycle (Georgetown, Tex.). 2014 Apr 1; 13 (7). 1049-50
Frank AO , Vangamudi B , Feldkamp MD , Souza-Fagundes EM , Luzwick JW , Cortez D , Olejniczak ET , Waterson AG , Rossanese OW , Chazin WJ , Fesik SW , Discovery of a potent stapled helix peptide that binds to the 70N domain of replication protein A. Journal of medicinal chemistry. 2014 Mar 27; 57 (6). 2455-61
Carroll C , Bansbach CE , Zhao R , Jung SY , Qin J , Cortez D , Phosphorylation of a C-terminal auto-inhibitory domain increases SMARCAL1 activity. Nucleic acids research. 2014 Jan ; 42 (2). 918-25

2013

Nagarajan P , Ge Z , Sirbu B , Doughty C , Agudelo Garcia PA , Schlederer M , Annunziato AT , Cortez D , Kenner L , Parthun MR , Histone acetyl transferase 1 is essential for mammalian development, genome stability, and the processing of newly synthesized histones H3 and H4. PLoS genetics. 2013 Jun ; 9 (6). e1003518
Summers AR , Fischer MA , Stengel KR , Zhao Y , Kaiser JF , Wells CE , Hunt A , Bhaskara S , Luzwick JW , Sampathi S , Chen X , Thompson MA , Cortez D , Hiebert SW , HDAC3 is essential for DNA replication in hematopoietic progenitor cells. The Journal of clinical investigation. 2013 Jul 1; 123 (7). 3112-23
Wells CE , Bhaskara S , Stengel KR , Zhao Y , Sirbu B , Chagot B , Cortez D , Khabele D , Chazin WJ , Cooper A , Jacques V , Rusche J , Eischen CM , McGirt LY , Hiebert SW , Inhibition of histone deacetylase 3 causes replication stress in cutaneous T cell lymphoma. PloS one. 2013 ; 8 (7). e68915
Couch FB , Bansbach CE , Driscoll R , Luzwick JW , Glick GG , Bétous R , Carroll CM , Jung SY , Qin J , Cimprich KA , Cortez D , ATR phosphorylates SMARCAL1 to prevent replication fork collapse. Genes & development. 2013 Jul 15; 27 (14). 1610-23
Sirbu BM , Cortez D , DNA damage response: three levels of DNA repair regulation. Cold Spring Harbor perspectives in biology. 2013 Aug ; 5 (8). a012724
Bétous R , Couch FB , Mason AC , Eichman BF , Manosas M , Cortez D , Substrate-selective repair and restart of replication forks by DNA translocases. Cell reports. 2013 Jun 27; 3 (6). 1958-69
Bétous R , Glick GG , Zhao R , Cortez D , Identification and characterization of SMARCAL1 protein complexes. PloS one. 2013 ; 8 (5). e63149
Carroll C , Badu-Nkansah A , Hunley T , Baradaran-Heravi A , Cortez D , Frangoul H , Schimke Immunoosseous Dysplasia associated with undifferentiated carcinoma and a novel SMARCAL1 mutation in a child. Pediatric blood & cancer. 2013 Sep ; 60 (9). E88-90

2012

Sirbu BM , Couch FB , Cortez D , Monitoring the spatiotemporal dynamics of proteins at replication forks and in assembled chromatin using isolation of proteins on nascent DNA. Nature protocols. 2012 Mar ; 7 (3). 594-605
Bétous R , Mason AC , Rambo RP , Bansbach CE , Badu-Nkansah A , Sirbu BM , Eichman BF , Cortez D , SMARCAL1 catalyzes fork regression and Holliday junction migration to maintain genome stability during DNA replication. Genes & development. 2012 Jan 15; 26 (2). 151-62

2011

Bansbach CE , Boerkoel CF , Cortez D , SMARCAL1 and replication stress: an explanation for SIOD? Nucleus (Austin, Tex.). 2011 ; 1 (3). 245-8
Nam EA , Zhao R , Cortez D , Analysis of mutations that dissociate G(2) and essential S phase functions of human ataxia telangiectasia-mutated and Rad3-related (ATR) protein kinase. The Journal of biological chemistry. 2011 Oct 28; 286 (43). 37320-7
Bansbach CE , Cortez D , Defining genome maintenance pathways using functional genomic approaches. Critical reviews in biochemistry and molecular biology. 2011 Aug ; 46 (4). 327-41
Nam EA , Zhao R , Glick GG , Bansbach CE , Friedman DB , Cortez D , Thr-1989 phosphorylation is a marker of active ataxia telangiectasia-mutated and Rad3-related (ATR) kinase. The Journal of biological chemistry. 2011 Aug 19; 286 (33). 28707-14
Sirbu BM , Couch FB , Feigerle JT , Bhaskara S , Hiebert SW , Cortez D , Analysis of protein dynamics at active, stalled, and collapsed replication forks. Genes & development. 2011 Jun 15; 25 (12). 1320-7
Nam EA , Cortez D , ATR signalling: more than meeting at the fork. The Biochemical journal. 2011 Jun 15; 436 (3). 527-36

2010

Yu DS , Zhao R , Hsu EL , Cayer J , Ye F , Guo Y , Shyr Y , Cortez D , Cyclin-dependent kinase 9-cyclin K functions in the replication stress response. EMBO reports. 2010 Nov ; 11 (11). 876-82
Wiltshire TD , Lovejoy CA , Wang T , Xia F , O'Connor MJ , Cortez D , Sensitivity to poly(ADP-ribose) polymerase (PARP) inhibition identifies ubiquitin-specific peptidase 11 (USP11) as a regulator of DNA double-strand break repair. The Journal of biological chemistry. 2010 May 7; 285 (19). 14565-71

2009

Lovejoy CA , Xu X , Bansbach CE , Glick GG , Zhao R , Ye F , Sirbu BM , Titus LC , Shyr Y , Cortez D , Functional genomic screens identify CINP as a genome maintenance protein. Proceedings of the National Academy of Sciences of the United States of America. 2009 Nov 17; 106 (46). 19304-9
Bansbach CE , Bétous R , Lovejoy CA , Glick GG , Cortez D , The annealing helicase SMARCAL1 maintains genome integrity at stalled replication forks. Genes & development. 2009 Oct 15; 23 (20). 2405-14
Nam EA , Cortez D , SOSS1/2: Sensors of single-stranded DNA at a break. Molecular cell. 2009 Aug 14; 35 (3). 258-9
Lovejoy CA , Cortez D , Common mechanisms of PIKK regulation. DNA repair. 2009 Sep 2; 8 (9). 1004-8

2008

Cimprich KA , Cortez D , ATR: an essential regulator of genome integrity. Nature reviews. Molecular cell biology. 2008 Aug ; 9 (8). 616-27
Mordes DA , Nam EA , Cortez D , Dpb11 activates the Mec1-Ddc2 complex. Proceedings of the National Academy of Sciences of the United States of America. 2008 Dec 2; 105 (48). 18730-4
Xu X , Vaithiyalingam S , Glick GG , Mordes DA , Chazin WJ , Cortez D , The basic cleft of RPA70N binds multiple checkpoint proteins, including RAD9, to regulate ATR signaling. Molecular and cellular biology. 2008 Dec ; 28 (24). 7345-53
Mordes DA , Cortez D , Activation of ATR and related PIKKs. Cell cycle (Georgetown, Tex.). 2008 Sep 15; 7 (18). 2809-12
Mordes DA , Glick GG , Zhao R , Cortez D , TopBP1 activates ATR through ATRIP and a PIKK regulatory domain. Genes & development. 2008 Jun 1; 22 (11). 1478-89
Bhaskara S , Chyla BJ , Amann JM , Knutson SK , Cortez D , Sun ZW , Hiebert SW , Deletion of histone deacetylase 3 reveals critical roles in S phase progression and DNA damage control. Molecular cell. 2008 Apr 11; 30 (1). 61-72

2007

Myers JS , Zhao R , Xu X , Ham AJ , Cortez D , Cyclin-dependent kinase 2 dependent phosphorylation of ATRIP regulates the G2-M checkpoint response to DNA damage. Cancer research. 2007 Jul 15; 67 (14). 6685-90
Chen X , Zhao R , Glick GG , Cortez D , Function of the ATR N-terminal domain revealed by an ATM/ATR chimera. Experimental cell research. 2007 May 1; 313 (8). 1667-74
Ball HL , Ehrhardt MR , Mordes DA , Glick GG , Chazin WJ , Cortez D , Function of a conserved checkpoint recruitment domain in ATRIP proteins. Molecular and cellular biology. 2007 May ; 27 (9). 3367-77

2006

Lovejoy CA , Lock K , Yenamandra A , Cortez D , DDB1 maintains genome integrity through regulation of Cdt1. Molecular and cellular biology. 2006 Nov ; 26 (21). 7977-90
Myers JS , Cortez D , Rapid activation of ATR by ionizing radiation requires ATM and Mre11. The Journal of biological chemistry. 2006 Apr 7; 281 (14). 9346-50

2005

Ball HL , Cortez D , ATRIP oligomerization is required for ATR-dependent checkpoint signaling. The Journal of biological chemistry. 2005 Sep 9; 280 (36). 31390-6
Cortez D , Unwind and slow down: checkpoint activation by helicase and polymerase uncoupling. Genes & development. 2005 May 1; 19 (9). 1007-12
Ball HL , Myers JS , Cortez D , ATRIP binding to replication protein A-single-stranded DNA promotes ATR-ATRIP localization but is dispensable for Chk1 phosphorylation. Molecular biology of the cell. 2005 May ; 16 (5). 2372-81

2004

Cortez D , Glick G , Elledge SJ , Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases. Proceedings of the National Academy of Sciences of the United States of America. 2004 Jul 6; 101 (27). 10078-83

2003

Cortez D , Caffeine inhibits checkpoint responses without inhibiting the ataxia-telangiectasia-mutated (ATM) and ATM- and Rad3-related (ATR) protein kinases. The Journal of biological chemistry. 2003 Sep 26; 278 (39). 37139-45